Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Tuner (DE3) cells | Enterovirus D68 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.07 M sodium acetate (pH 4.6), 5-15% (w/v) PEG 4000, and 10-30% (v/v) glycerol | Enterovirus D68 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-amino-6-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-6-methyl-4-oxoheptanoic acid | - |
Enterovirus D68 | |
4-amino-7-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-7-methyl-5-oxooctanoic acid | - |
Enterovirus D68 | |
ethyl (2E,4S)-4-([N-[(benzyloxy)carbonyl]-L-phenylalanyl]amino)-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | - |
Enterovirus D68 | |
ethyl (2E,4S)-4-[(N-[5-[(tert-butoxycarbonyl)amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl]-L-phenylalanyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | - |
Enterovirus D68 | |
ethyl (2E,4S)-4-[(tert-butoxycarbonyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | - |
Enterovirus D68 | |
ethyl (2E,4S)-4-[[N-(5-amino-6-hydroxy-2-methyl-4-oxohexan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | most potent inhibitor | Enterovirus D68 | |
ethyl (2E,4S)-4-[[N-(5-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | - |
Enterovirus D68 | |
ethyl (2E,4S)-4-[[N-(tert-butoxycarbonyl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate | - |
Enterovirus D68 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus D68 | - |
- |
- |
Enterovirus D68 3799 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Enterovirus D68 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KEALFQGPPQFE-Edans + H2O | best substrate | Enterovirus D68 | Dabcyl-KEALFQ + GPPQFE-Edans | - |
? | |
Dabcyl-KEALFQGPPQFE-Edans + H2O | best substrate | Enterovirus D68 3799 | Dabcyl-KEALFQ + GPPQFE-Edans | - |
? | |
Dabcyl-TAKVQGPGFD-Edans + H2O | - |
Enterovirus D68 | Dabcyl-TAKVQ + GPGFD-Edans | - |
? | |
Dabcyl-TAKVQGPGFD-Edans + H2O | - |
Enterovirus D68 3799 | Dabcyl-TAKVQ + GPGFD-Edans | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Enterovirus D68 |
3Cpro | - |
Enterovirus D68 |