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Literature summary for 3.4.22.28 extracted from

  • Tan, J.; George, S.; Kusov, Y.; Perbandt, M.; Anemueller, S.; Mesters, J.R.; Norder, H.; Coutard, B.; Lacroix, C.; Leyssen, P.; Neyts, J.; Hilgenfeld, R.
    3C protease of enterovirus 68: structure-based design of Michael acceptor inhibitors and their broad-spectrum antiviral effects against picornaviruses (2013), J. Virol., 87, 4339-4351.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Enterovirus D68

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.07 M sodium acetate (pH 4.6), 5-15% (w/v) PEG 4000, and 10-30% (v/v) glycerol Enterovirus D68

Inhibitors

Inhibitors Comment Organism Structure
3-amino-6-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-6-methyl-4-oxoheptanoic acid
-
 Enterovirus D68
4-amino-7-([[(2S)-1-([(2S,3E)-5-ethoxy-5-oxo-1-[(3S)-2-oxopyrrolidin-3-yl]pent-3-en-2-yl]amino)-1-oxo-3-phenylpropan-2-yl]amino]oxy)-7-methyl-5-oxooctanoic acid
-
 Enterovirus D68
ethyl (2E,4S)-4-([N-[(benzyloxy)carbonyl]-L-phenylalanyl]amino)-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
 Enterovirus D68
ethyl (2E,4S)-4-[(N-[5-[(tert-butoxycarbonyl)amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl]-L-phenylalanyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
 Enterovirus D68
ethyl (2E,4S)-4-[(tert-butoxycarbonyl)amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
 Enterovirus D68
ethyl (2E,4S)-4-[[N-(5-amino-6-hydroxy-2-methyl-4-oxohexan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate most potent inhibitor Enterovirus D68
ethyl (2E,4S)-4-[[N-(5-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]-6-hydroxy-2-methyl-4-oxoheptan-2-yl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
 Enterovirus D68
ethyl (2E,4S)-4-[[N-(tert-butoxycarbonyl)-L-phenylalanyl]amino]-5-[(3S)-2-oxopyrrolidin-3-yl]pent-2-enoate
-
 Enterovirus D68

Organism

Organism UniProt Comment Textmining
Enterovirus D68
-
-
-
Enterovirus D68 3799
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Enterovirus D68

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-KEALFQGPPQFE-Edans + H2O best substrate Enterovirus D68 Dabcyl-KEALFQ + GPPQFE-Edans
-
 ?
Dabcyl-KEALFQGPPQFE-Edans + H2O best substrate Enterovirus D68 3799 Dabcyl-KEALFQ + GPPQFE-Edans
-
 ?
Dabcyl-TAKVQGPGFD-Edans + H2O
-
 Enterovirus D68 Dabcyl-TAKVQ + GPGFD-Edans
-
 ?
Dabcyl-TAKVQGPGFD-Edans + H2O
-
 Enterovirus D68 3799 Dabcyl-TAKVQ + GPGFD-Edans
-
 ?

Synonyms

Synonyms Comment Organism
3C protease
-
 Enterovirus D68
3Cpro
-
 Enterovirus D68