Crystallization (Comment) | Organism |
---|---|
X-ray structure of the Southampton norovirus 3C protease (SV3CP) bound to an active site-directed peptide inhibitor which has been refined at 1.7 A resolution is described | Southampton virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetyl-L-alpha-glutamyl-L-phenylalanyl-L-glutaminyl-N-[(2E,4S)-7-amino-1-ethoxy-1,7-dioxohept-2-en-4-yl]-L-leucinamide | i.e. Michael acceptor peptidyl inhibitor (MAPI). Irreversible inhibitor in which the natural polyprotein substrate recognition sequence is linked to a propenyl ethyl ester moiety (X). The catalytic cysteine at position 139 of the enzyme is rapidly modified by the inhibitor | Southampton virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Ac-EFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
0.8 | - |
Ac-DEFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
1.5 | - |
Ac-FQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Southampton virus | Q04544 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-DEFQLQ-4-nitroanilide + H2O | - |
Southampton virus | ? | - |
? | |
Ac-EFQLQ-4-nitroanilide + H2O | - |
Southampton virus | ? | - |
? | |
Ac-FQLQ-4-nitroanilide + H2O | - |
Southampton virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Southampton virus |
SV3CP | - |
Southampton virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
Ac-FQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
0.14 | - |
Ac-EFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
0.33 | - |
Ac-DEFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Southampton virus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Ac-FQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
0.41 | - |
Ac-DEFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus | |
0.46 | - |
Ac-EFQLQ-4-nitroanilide | pH 8.5, temperature not specified in the publication | Southampton virus |