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Literature summary for 3.4.22.28 extracted from
- Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases (2008), Virology, 378, 118-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in HEK-293T cells | Enterovirus C |
| expressed in HEK-293T cells | coxsackievirus |
| recombinant 3Cpro is expressed in Escherichia coli | Enterovirus C |
| recombinant 3Cpro is expressed in Escherichia coli | coxsackievirus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| eukaryotic initiation factor + H2O | coxsackievirus | cleaveage at a single site, rabbit eIF5B is proteolytically cleaves during coxsackievirus infection of cultured cells, beginning at 3 h post-infection and increasing thereafter | eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-? | - |
? |  |
| eukaryotic initiation factor + H2O | Enterovirus C | cleaveage at a single site, rabbit eIF5B is proteolytically cleaves during poliovirus infection of cultured cells, beginning at 3 hours post-infection and increasing thereafter | eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| coxsackievirus | - |
Coxsackie B virus (CVB) | - |
| Enterovirus C | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterovirus C |
- |
coxsackievirus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | recombinant HRV14 3Cpro | coxsackievirus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| eukaryotic initiation factor + H2O | cleaveage at a single site, rabbit eIF5B is proteolytically cleaves during coxsackievirus infection of cultured cells, beginning at 3 h post-infection and increasing thereafter | coxsackievirus | eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-? | - |
? | |
| eukaryotic initiation factor + H2O | cleaveage at a single site, rabbit eIF5B is proteolytically cleaves during poliovirus infection of cultured cells, beginning at 3 hours post-infection and increasing thereafter | Enterovirus C | eukaryotic initiation factor 1-478 + eukaryotic initiation factor 479-? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3C protease | - |
Enterovirus C |
| 3C protease | - |
coxsackievirus |
| 3Cpro | - |
Enterovirus C |
| 3Cpro | - |
coxsackievirus |
| picornain 3C | - |
Enterovirus C |
| picornain 3C | - |
coxsackievirus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, 150 mM NaCl, 50 mM Tris-HCl | Enterovirus C |
| 7.5 | - |
assay at, 150 mM NaCl, 50 mM Tris-HCl | coxsackievirus |
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