Application | Comment | Organism |
---|---|---|
medicine | 3Cpro as an attractive target for antiviral drugs | Foot-and-mouth disease virus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) pLysS from a pET-28 vector modified to add a thrombin-cleavable N-terminal polyhistidine tag | Foot-and-mouth disease virus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion, at 1.9 A resolution | Foot-and-mouth disease virus |
Protein Variants | Comment | Organism |
---|---|---|
C142S | enhanced solubility | Foot-and-mouth disease virus |
C163A | elimination of the activity of the active site nucleophile | Foot-and-mouth disease virus |
C95K | enhanced solubility | Foot-and-mouth disease virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Foot-and-mouth disease virus | - |
- |
- |
Foot-and-mouth disease virus A10_61 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on TALON resin and by gel filtration on a Hi Load 16/60 Superdex 75 column | Foot-and-mouth disease virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Foot-and-mouth disease virus polyprotein + H2O | 3Cpro is only weakly selective for Gln over Glu, most susceptible peptides correspond to the VP1/2A and 2B/2C junctions, which were completely cleaved within 6 hours, whereas peptides corresponding to the VP2/VP3, 2C/3A, 3A/3B1, 3B1/3B2 and 3B2/3B3 junctions were uncleaved after 24 hours incubation | Foot-and-mouth disease virus | ? | - |
? | |
Foot-and-mouth disease virus polyprotein + H2O | 3Cpro is only weakly selective for Gln over Glu, most susceptible peptides correspond to the VP1/2A and 2B/2C junctions, which were completely cleaved within 6 hours, whereas peptides corresponding to the VP2/VP3, 2C/3A, 3A/3B1, 3B1/3B2 and 3B2/3B3 junctions were uncleaved after 24 hours incubation | Foot-and-mouth disease virus A10_61 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3C protease | - |
Foot-and-mouth disease virus |
3Cpro | - |
Foot-and-mouth disease virus |