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Literature summary for 3.4.22.28 extracted from

  • Birtley, J.R.; Curry, S.
    Crystallization of foot-and-mouth disease virus 3C protease: surface mutagenesis and a novel crystal-optimization strategy (2005), Acta Crystallogr. Sect. D, 61, 646-650.
    View publication on PubMed

Application

Application Comment Organism
medicine foot-and mouth disease virus 3Cpro plays a vital role in virus replication, the enzyme is an attractive target for antiviral drugs Foot-and-mouth disease virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) pLysS Foot-and-mouth disease virus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion, iterative crystal-screening strategy, at 1.9 A resolution Foot-and-mouth disease virus

Protein Variants

Protein Variants Comment Organism
A133S processed enzyme, polydispersity of 35% Foot-and-mouth disease virus
A133S/C142S processed enzyme, polydispersity of 29% Foot-and-mouth disease virus
C142S processed enzyme, polydispersity of 25% Foot-and-mouth disease virus
C95K processed enzyme, polydispersity of 37% Foot-and-mouth disease virus
C95K/C142S processed enzyme, polydispersity of 11% Foot-and-mouth disease virus
F70S incorrect processed enzyme Foot-and-mouth disease virus
I55D insoluble enzyme Foot-and-mouth disease virus
I55N/M56T incorrect processed enzyme Foot-and-mouth disease virus
L82D processed enzyme, polydispersity of 55% Foot-and-mouth disease virus
L82D/C142S partially processed enzyme, polydispersity of 33% Foot-and-mouth disease virus
M81N processed enzyme, polydispersity of 33% Foot-and-mouth disease virus
M81N/C142S processed enzyme, polydispersity of 19% Foot-and-mouth disease virus
P114S/V115D unprocessed enzyme Foot-and-mouth disease virus
V124S processed enzyme, polydispersity of 47% Foot-and-mouth disease virus
V124S/C142S processed enzyme, polydispersity of 29% Foot-and-mouth disease virus
V140S processed enzyme Foot-and-mouth disease virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
-
Foot-and-mouth disease virus
25000
-
-
Foot-and-mouth disease virus

Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Foot-and-mouth disease virus

Synonyms

Synonyms Comment Organism
3C protease
-
Foot-and-mouth disease virus
3Cpro
-
Foot-and-mouth disease virus