Cloned (Comment) | Organism |
---|---|
the cathepsin S propeptide is expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cathepsin S propeptide | 0.0002 mM, blocks gelatinase, collagenase and elastinase activities of mature cathepsin S | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
His-tagged recombinant propeptide, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ HiTrap column chromatography | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
complete removal of urea phosphate buffer starting from 8 M | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
Collagen + H2O | - |
Homo sapiens | ? | - |
? | |
Elastin + H2O | - |
Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CatS | mature enzyme | Homo sapiens |
CatSPP | cathepsin S propeptide | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000025 | - |
cathepsin S propeptide | using benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin as a substrate | Homo sapiens |