Literature summary for 3.4.22.27 extracted from

Kopitar, G.; Dolinar, M.; Strukelj, B.; Pungercar, B.; Turk, V.
Folding and activation of human procathepsin S from inclusion bodies produced in Escherichia coli (1996), Eur. J. Biochem., 236, 558-562.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
inclusion body	recombinant enzyme expressed in Escherichia coli	Homo sapiens	16234	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the proenzyme is autocatalytically processed to active cathepsin S at pH 4.5 in the presence of an excess of cysteine and catalytic amounts of dextran sulfate	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant procathepsin S from inclusion bodies produced in Escherichia coli is renatured at pH 7.6 by a two-step dilution	Homo sapiens

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Homo sapiens
More	most of the loss of procathepsin S occurs during folding, probably because of aggregation. Concentrations lower than 0.02 mg/ml of procathepsin S are necessary to minimise such aggregation	Homo sapiens

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Release 2023.1 (January 2023)