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Literature summary for 3.4.22.27 extracted from
- Cathepsin S from bovine spleen. Purification, distribution, intracellular localization and action on proteins (1989), Biochem. J., 264, 467-473.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is partially destroyed by autolysis of the homogenate at pH 4.2 | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic vesicle | - |
Bos taurus | 31410 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Bos taurus |
| 23700 | - |
x * 23700, calculation from nucleotide sequence | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme is expected to contain carbohydrate | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | concentrated to the cells of the proximal tubule | Bos taurus | - |
| lung | - |
Bos taurus | - |
| lymph node | - |
Bos taurus | - |
| spleen | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.582 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, 0.012-0.04 mM cathepsin S, 100 mM sodium acetate buffer, pH 5.5, 1 mM EDTA, stable for 8 months. In presence of 0.5 mM Hg2+ and 1 mM EDTA rapid decrease of about 50% of the activity which then remains unchanged for 3 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Albumin + H2O | - |
Bos taurus | ? | - |
? |  |
| azocasein + H2O | high activity in presence of urea at pH 5.5 | Bos taurus | ? | - |
? | |
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| Collagen + H2O | - |
Bos taurus | ? | - |
? | |
| haemoglobin + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23700, calculation from nucleotide sequence | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 5.5-7.0, in presence for dithiothreitol and EDTA stable for 240 min. 10-12% of the enzyme activity is lost after 60 min at pH 7.5 or pH 4.0. In absence of dithiothreitol and EDTA the enzyme is inactivated at pH 4.0 by 70% after 60 min | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA, 70% inactivation after 60 min in absence of dithiothreitol and EDTA | Bos taurus |
| 5.5 | 7 | 40°C, stable for 240 min in presence of dithiothreitol and EDTA | Bos taurus |
| 7.5 | - |
40°C, 10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA | Bos taurus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bos taurus | isoelectric focusing | - |
7 |
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