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Literature summary for 3.4.22.27 extracted from
- Cloning, expression, purification, and activity of dog (Canis familiaris) and monkey (Saimiri boliviensis) cathepsin S (2003), Protein Expr. Purif., 28, 93-101.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Canis lupus familiaris |
- |
Saimiri boliviensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Bos taurus |  |
| 0.011 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Mus musculus | |
| 0.013 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Canis lupus familiaris | |
| 0.014 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Homo sapiens | |
| 0.024 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Saimiri boliviensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Canis lupus familiaris |
| 26000 | - |
x * 26000, SDS-PAGE | Saimiri boliviensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | - |
? | - |
? | |
| additional information | Homo sapiens | - |
? | - |
? | |
| additional information | Canis lupus familiaris | key protease responsible for the removal of the invariant chain from MHC class II molecules, the enzyme plays a major role in antigen presentation | ? | - |
? | |
| additional information | Saimiri boliviensis | key protease responsible for the removal of the invariant chain from MHC class II molecules, the enzyme plays a major role in antigen presentation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Canis lupus familiaris | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Saimiri boliviensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Canis lupus familiaris |
- |
Saimiri boliviensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cathepsin S is not expressed in HeLa-CIITA and MeIJuSo cells | Saimiri boliviensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Canis lupus familiaris | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Saimiri boliviensis | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| additional information | - |
Mus musculus | ? | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | key protease responsible for the removal of the invariant chain from MHC class II molecules, the enzyme plays a major role in antigen presentation | Canis lupus familiaris | ? | - |
? | |
| additional information | key protease responsible for the removal of the invariant chain from MHC class II molecules, the enzyme plays a major role in antigen presentation | Saimiri boliviensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
| ? | - |
Bos taurus |
| ? | x * 26000, SDS-PAGE | Canis lupus familiaris |
| ? | x * 26000, SDS-PAGE | Saimiri boliviensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.4 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Canis lupus familiaris | |
| 15.1 | - |
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Bos taurus | |
| 20.3 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Mus musculus | |
| 20.3 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Homo sapiens | |
| 33.1 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | pH 5.0, room temperature | Saimiri boliviensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
and a second lower pH-optimum at pH 8.0 | Mus musculus |
| 5.5 | - |
and a second lower pH-optimum at pH 8.0 | Canis lupus familiaris |
| 5.5 | - |
hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Mus musculus |
| 5.5 | - |
hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Canis lupus familiaris |
| 6 | - |
hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Homo sapiens |
| 6.5 | - |
hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Saimiri boliviensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | pH 3.5: about 60% of maximal activity, pH 7.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Saimiri boliviensis |
| 3.5 | 8.5 | pH 3.5: about 55% of maximal activity, pH 7.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, pH 8.5: about 35% of maximal activity, hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Mus musculus |
| 3.5 | 8.5 | pH 3.5: about 50% of maximal activity, pH 7.5: about 40% of maximal activity, pH 8.0: about 85% of maximal activity, pH 8.5: about 30% of maximal activity, hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Canis lupus familiaris |
| 4.5 | 8 | - |
Bos taurus |
| 4.5 | 8 | pH 4.5: about 35% of maximal activity, pH 7.5: about 40% of maximal activity, pH 8.0: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | Homo sapiens |
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