BRENDA - Enzyme Database show
show all sequences of 3.4.22.26

Cancer procoagulant activity studies using synthetic peptidyl substrates

Mielicki, W.P.; Mielicka, E.; Gordon, S.G.; Thromb. Res. 87, 251-256 (1997)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
enzyme has potential as a sensitive and early tumor marker
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
-
Homo sapiens
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
ie.e. E-64
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
D-Ile-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.061
-
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.109
-
D-Ala-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.153
-
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
pH 7.2
Homo sapiens
0.306
-
Glu-Glu-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
factor X + H2O
Homo sapiens
cleavage of Arg-Ile bond, activation of the coaglutation factor X
factor Xa
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
amnion
-
Homo sapiens
-
chorion
-
Homo sapiens
-
additional information
produced by malignant and fetal cells
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
47
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
benzyloxycarbonyl-Leu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Leu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
benzyloxycarbonyl-Val-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Val-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Ala-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Ala-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Ile-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Ile-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Phe-Pip-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Phe-Pip-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
factor X + H2O
cleavage of Arg-Ile bond
653983
Homo sapiens
factor Xa
-
653983
Homo sapiens
?
factor X + H2O
cleavage of Arg-Ile bond, activation of the coaglutation factor X
653983
Homo sapiens
factor Xa
-
653983
Homo sapiens
?
Fibrinogen + H2O
-
653983
Homo sapiens
?
-
653983
Homo sapiens
?
Glu-Glu-Phe-Lys-4-nitroanilide + H2O
-
653983
Homo sapiens
Glu-Glu-Phe-Lys + 4-nitroaniline
-
653983
Homo sapiens
?
Glu-Glu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Glu-Glu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Leu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Leu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
additional information
substrate specificity, overview
653983
Homo sapiens
?
-
653983
Homo sapiens
?
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
N-p-tosyl-Gly-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
653983
Homo sapiens
N-p-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
653983
Homo sapiens
?
sarcosyl-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
sarcosyl-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Val-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Val-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0073
-
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0082
-
D-Ile-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0119
-
Glu-Glu-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0129
-
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
pH 7.2
Homo sapiens
0.0132
-
D-Ala-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Homo sapiens
Application (protein specific)
Application
Commentary
Organism
medicine
enzyme has potential as a sensitive and early tumor marker
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
-
Homo sapiens
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
ie.e. E-64
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
D-Ile-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.061
-
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.109
-
D-Ala-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.153
-
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
pH 7.2
Homo sapiens
0.306
-
Glu-Glu-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
factor X + H2O
Homo sapiens
cleavage of Arg-Ile bond, activation of the coaglutation factor X
factor Xa
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
amnion
-
Homo sapiens
-
chorion
-
Homo sapiens
-
additional information
produced by malignant and fetal cells
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
47
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
benzyloxycarbonyl-Leu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Leu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
benzyloxycarbonyl-Val-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
benzyloxycarbonyl-Val-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Ala-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Ala-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Ile-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Ile-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
D-Phe-Pip-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
D-Phe-Pip-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
factor X + H2O
cleavage of Arg-Ile bond
653983
Homo sapiens
factor Xa
-
653983
Homo sapiens
?
factor X + H2O
cleavage of Arg-Ile bond, activation of the coaglutation factor X
653983
Homo sapiens
factor Xa
-
653983
Homo sapiens
?
Fibrinogen + H2O
-
653983
Homo sapiens
?
-
653983
Homo sapiens
?
Glu-Glu-Phe-Lys-4-nitroanilide + H2O
-
653983
Homo sapiens
Glu-Glu-Phe-Lys + 4-nitroaniline
-
653983
Homo sapiens
?
Glu-Glu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Glu-Glu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Leu-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Leu-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
methylsulfonyl-D-cyclohexylalanyl-butyl-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
additional information
substrate specificity, overview
653983
Homo sapiens
?
-
653983
Homo sapiens
?
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
N-p-tosyl-Gly-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
653983
Homo sapiens
N-p-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
653983
Homo sapiens
?
sarcosyl-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
sarcosyl-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Val-Pro-Arg-4-nitroanilide + H2O
-
653983
Homo sapiens
Val-Pro-Arg + 4-nitroaniline
-
653983
Homo sapiens
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0073
-
N-p-tosyl-Gly-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0082
-
D-Ile-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0119
-
Glu-Glu-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
0.0129
-
N-p-tosyl-Gly-Pro-Lys-4-nitroanilide
pH 7.2
Homo sapiens
0.0132
-
D-Ala-Pro-Arg-4-nitroanilide
pH 7.2
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Homo sapiens
Other publictions for EC 3.4.22.26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733446
Kee
The effect of cancer procoagul ...
Homo sapiens
Biol. Chem.
393
113-121
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
701292
Musallam
Incidence and prophylaxis of v ...
Homo sapiens
Thromb. Res.
123
679-686
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710603
Kaplinska
Cancer procoagulant (CP) analy ...
Homo sapiens
Thromb. Res.
124
364-367
2009
-
-
-
-
-
-
2
-
1
2
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
2
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697471
Kamocka
Rat prostate tumors express ca ...
Rattus norvegicus
Comp. Med.
58
282-286
2008
-
-
-
-
-
-
2
-
-
-
-
2
-
3
-
-
1
-
-
2
11
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
2
-
-
-
1
-
2
11
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
679297
Kamocka
Effect of immunisation with ca ...
Homo sapiens
Clin. Exp. Med.
6
119-123
2006
-
1
-
-
-
-
1
-
-
1
-
-
-
4
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679638
Szajda
Analysis of the relationship b ...
Homo sapiens
Eur. J. Gynaecol. Oncol.
27
495-499
2006
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668075
Kazmierczak
Cancer procoagulant in patient ...
Homo sapiens
Blood Coagul. Fibrinolysis
16
543-547
2005
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
4
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
4
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668137
Kamocka
Effect of cancer procoagulant ...
Homo sapiens
Cancer Lett.
222
89-94
2005
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
1
-
-
3
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
3
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668476
Jozwik
The activity of cancer procoag ...
Homo sapiens
Eur. J. Gynaecol. Oncol.
26
407-410
2005
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
7
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
7
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667302
Raasi
Properties of proteins in canc ...
Homo sapiens
Arch. Biochem. Biophys.
428
131-135
2004
-
-
-
-
-
-
2
-
-
2
-
1
-
1
-
-
1
-
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651435
Mielicki
Biochemistry of cancer procoag ...
Homo sapiens, Oryctolagus cuniculus
Haemostasis
31 Suppl 1
8-10
2001
8
-
-
-
-
-
24
-
1
2
1
4
-
2
-
2
1
-
-
6
-
-
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