Any feedback?
Literature summary for 3.4.22.2 extracted from
- The papain-catalyzed synthesis of hippuryl anilide (1964), Biochim. Biophys. Acta, 85, 446-461.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptopropionic acid | required, complete activation at 0.05 N when the enzyme concentration is 0.49 mM | Carica papaya |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldisulfide-beta,beta'-dicarboxylic acid | - |
Carica papaya | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hippuric acid + aniline | weak activity, 0.1% of the hydrolytic activity with N-benzoyl-L-argininamide | Carica papaya | hippuryl anilide | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is stable to heat in the oxidized, inactive form, activity is rapidly lost in presence of 2-mercaptopropionic acid, hippuric acid alters heat stability | Carica papaya |
| 50 | - |
oxidized, inactive enzyme, in absence of activator, 67% of the initial activity remains after 28 days | Carica papaya |
| 65 | - |
pH 5.0, complete loss of activity after 7 days | Carica papaya |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
