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BRENDA support

Literature summary for 3.4.22.2 extracted from

  • Li, M.; Su, E.; You, P.; Gong, X.; Sun, M.; Xu, D.; Wei, D.
    Purification and in situ immobilization of papain with aqueous two-phase system (2010), PLoS ONE, 5, e15168.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Carica papaya
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Purification (Commentary)

Purification (Comment) Organism
aqueous two-phase system (40% (w/w) 15 mg/ml enzyme solution, 14.33-17.65% (w/w) PEG 6000, 14.27-14.42% (w/w) NaH2PO4/K2HPO4 and pH 5.77-6.3 at 20°C). Propanol generates solid enzyme aggregates with almost 120% activity upon resolubilization through dilution of the precipitant Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
latex
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Carica papaya
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
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Carica papaya Nalpha-benzoyl-DL-arginine + 4-nitroaniline
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