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Literature summary for 3.4.22.2 extracted from

  • Choudhury, D.; Biswas, S.; Roy, S.; Dattagupta, J.K.
    Improving thermostability of papain through structure-based protein engineering (2010), Protein Eng. Des. Sel., 23, 457-467.
    View publication on PubMed
Search for more literature for 3.4.22.2

Protein Variants

Protein Variants Comment Organism
K174R mutation introduced according to thermostable homologue ervatamin C, unstable Carica papaya
K174R/V32S mutation introduced according to thermostable homologue ervatamin C, improvement of thermal stability Carica papaya
K174R/V32S/G36S mutation introduced according to thermostable homologue ervatamin C, improvement of thermal stability Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
121
-
 mutant K174R/V32S, pH 6.5, 37°C Carica papaya
128
-
 wild-type, pH 6.5, 37°C Carica papaya
130
-
 mutant K174R/V32S/G36S, pH 6.5, 37°C Carica papaya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Carica papaya ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 wild-type Carica papaya
60
-
 mutant K174R/V32S Carica papaya
65
-
 mutant K174R/V32S/G36S Carica papaya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 77, 114, and 171 min, respectively Carica papaya
65
-
 half-life of wild-type, mutant K174R/V32S and mutant K174R/V32S/G36S is 35, 45, and 80 min, respectively Carica papaya