Literature summary for 3.4.22.2 extracted from

Sathish, H.A.; Kumar, P.R.; Prakash, V.
Mechanism of solvent induced thermal stabilization of papain (2007), Int. J. Biol. Macromol., 41, 383-390.

General Stability

General Stability	Organism
in the presence of 20% (w/v) sorbitol the enzyme retains nearly 65% of its activity after heating at 75°C for 40 min and it retains almost 45% of its activity after heating for 80 min, the maximum effect is seen in the case of 40% (w/v) sorbitol, where the enzyme retains nearly 80 and 70% of its activity after heating at 75°C for 40 and 80 min, respectively, the enzyme also shows increased thermal stability in the presence of sucrose or xylose	Carica papaya

Organism

in the presence of 20% (w/v) sorbitol the enzyme retains nearly 65% of its activity after heating at 75°C for 40 min and it retains almost 45% of its activity after heating for 80 min, the maximum effect is seen in the case of 40% (w/v) sorbitol, where the enzyme retains nearly 80 and 70% of its activity after heating at 75°C for 40 and 80 min, respectively, the enzyme also shows increased thermal stability in the presence of sucrose or xylose

Carica papaya

Organic Solvent Stability

Organic Solvent	Comment	Organism
Glycerol	in the presence of 20% (v/v) glycerol the enzyme retains nearly 80% of its activity even after heating at 75°C for 40 min and it retains almost 70% of its activity even after heating for 80 min, at 30% (v/v) glycerol the enzyme retains nearly 80 and 65% of its activity after heating for 40 and 80 min, respectively at 75°C	Carica papaya

Organism

Organism	UniProt	Comment	Textmining
Carica papaya	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O	-	Carica papaya	N-alpha-benzoyl-DL-Arg + 4-nitroaniline	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
75	-	the native enzyme loses nearly 50% of its activity after heating at 75°C for 40 min, and it retains only 20% activity after heating for 80 min	Carica papaya

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Release 2023.1 (January 2023)