Application | Comment | Organism |
---|---|---|
biotechnology | papain immobilization on magnetic composite microspheres at pH 8.0 and 30°C, quantification and kinetics, overview, the immobilized enzyme exhibits better environmentally adaptability and reusability than the soluble enzyme | Carica papaya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and thermodynamics of soluble and immobilized enzyme at pH 7.0 and 40°C, overview | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Carica papaya | - |
Storage Stability | Organism |
---|---|
4°C, 60 days, the immobilized enzyme shows 20% loss of activity, the soluble enzyme shows 50% loss of activity | Carica papaya |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Carica papaya | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
soluble enzyme | Carica papaya |
80 | - |
immobilized enzyme | Carica papaya |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | temperature profile of soluble and immobilized enzyme | Carica papaya |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, the immobilized enzyme is completely stable, while the soluble enzyme loses activity | Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
soluble enzyme | Carica papaya |
8 | - |
immobilized enzyme | Carica papaya |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH profile of soluble and immobilized enzyme | Carica papaya |