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Literature summary for 3.4.22.16 extracted from

  • Matsuishi, M.; Saito, G.; Okitani, A.; Kato, H.
    Purification and some properties of cathepsin H from rabbit skeletal muscle (2003), Int. J. Biochem. Cell Biol., 35, 474-485.
    View publication on PubMed
Search for more literature for 3.4.22.16

Inhibitors

Inhibitors Comment Organism Structure
antipain at 0.1 mM, 95% inhibition Oryctolagus cuniculus
CPI-H peptide inhibitor of 13 kDa from rabbit skeletal muscle, noncompetitive Oryctolagus cuniculus
CPI-L peptide inhibitor of 23 kDa from rabbit skeletal muscle, noncompetitive Oryctolagus cuniculus
iodoacetic acid at 1 mM, 99% inhibition Oryctolagus cuniculus
leupeptin at 0.1 mM, 99% inhibition Oryctolagus cuniculus
additional information not inhibitory: pepstatin, bestatin, puromycin, phenylmethylsulfonylfluoride, trypsin inhibitor Oryctolagus cuniculus
TLCK
-
 Oryctolagus cuniculus
TPCK
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.203
-
 L-Leu-beta-naphthylamide pH 6.2, 37°C Oryctolagus cuniculus
0.367
-
 alpha-N-benzoyl-DL-Arg-beta-naphthylamide pH 6.2, 37°C Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Oryctolagus cuniculus
30000
-
 1 * 30000, SDS-PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle skeletal muscle Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0088
-
 pH 6.2, 37°C Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
actin + H2O degradation at pH 4.0 Oryctolagus cuniculus ?
-
 ?
alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus alpha-N-benzoyl-DL-Arg + beta-naphthylamine
-
 ?
Gly-beta-naphthylamide + H2O very poor substrate Oryctolagus cuniculus Gly + beta-naphthylamine
-
 ?
L-Ala-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Ala + beta-naphthylamine
-
 ?
L-Arg-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Arg + beta-naphthylamine
-
 ?
L-Leu-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Leu + beta-naphthylamine
-
 ?
L-Lys-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Lys + beta-naphthylamine
-
 ?
L-Met-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Met + beta-naphthylamine
-
 ?
L-Pro-beta-naphthylamide + H2O very poor substrate Oryctolagus cuniculus L-Pro + beta-naphthylamine
-
 ?
L-Ser-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus L-Ser + beta-naphthylamine
-
 ?
myosin heavy chain + H2O degradation at pH 4.0 Oryctolagus cuniculus ?
-
 ?
tropomyosin + H2O degradation at pH 4.0 Oryctolagus cuniculus ?
-
 ?
troponin I + H2O degradation at pH 4.0, little degradation at pH 5.o-5.6 Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 up to, almost stable Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 substrate alpha-N-benzoyl-DL-Arg-beta-naphthylamide Oryctolagus cuniculus
7
-
 L-Leu-beta-naphthylamide Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000033
-
 CPI-H pH 6.2, 30°C Oryctolagus cuniculus
0.0000005
-
 CPI-L pH 6.2, 30°C Oryctolagus cuniculus