Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | at 0.1 mM, 95% inhibition | Oryctolagus cuniculus | |
CPI-H | peptide inhibitor of 13 kDa from rabbit skeletal muscle, noncompetitive | Oryctolagus cuniculus | |
CPI-L | peptide inhibitor of 23 kDa from rabbit skeletal muscle, noncompetitive | Oryctolagus cuniculus | |
iodoacetic acid | at 1 mM, 99% inhibition | Oryctolagus cuniculus | |
leupeptin | at 0.1 mM, 99% inhibition | Oryctolagus cuniculus | |
additional information | not inhibitory: pepstatin, bestatin, puromycin, phenylmethylsulfonylfluoride, trypsin inhibitor | Oryctolagus cuniculus | |
TLCK | - |
Oryctolagus cuniculus | |
TPCK | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.203 | - |
L-Leu-beta-naphthylamide | pH 6.2, 37°C | Oryctolagus cuniculus | |
0.367 | - |
alpha-N-benzoyl-DL-Arg-beta-naphthylamide | pH 6.2, 37°C | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Oryctolagus cuniculus |
30000 | - |
1 * 30000, SDS-PAGE | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal muscle | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0088 | - |
pH 6.2, 37°C | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
actin + H2O | degradation at pH 4.0 | Oryctolagus cuniculus | ? | - |
? | |
alpha-N-benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | alpha-N-benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
Gly-beta-naphthylamide + H2O | very poor substrate | Oryctolagus cuniculus | Gly + beta-naphthylamine | - |
? | |
L-Ala-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Ala + beta-naphthylamine | - |
? | |
L-Arg-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Arg + beta-naphthylamine | - |
? | |
L-Leu-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Leu + beta-naphthylamine | - |
? | |
L-Lys-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Lys + beta-naphthylamine | - |
? | |
L-Met-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Met + beta-naphthylamine | - |
? | |
L-Pro-beta-naphthylamide + H2O | very poor substrate | Oryctolagus cuniculus | L-Pro + beta-naphthylamine | - |
? | |
L-Ser-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | L-Ser + beta-naphthylamine | - |
? | |
myosin heavy chain + H2O | degradation at pH 4.0 | Oryctolagus cuniculus | ? | - |
? | |
tropomyosin + H2O | degradation at pH 4.0 | Oryctolagus cuniculus | ? | - |
? | |
troponin I + H2O | degradation at pH 4.0, little degradation at pH 5.o-5.6 | Oryctolagus cuniculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
up to, almost stable | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
substrate alpha-N-benzoyl-DL-Arg-beta-naphthylamide | Oryctolagus cuniculus |
7 | - |
L-Leu-beta-naphthylamide | Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000033 | - |
CPI-H | pH 6.2, 30°C | Oryctolagus cuniculus | |
0.0000005 | - |
CPI-L | pH 6.2, 30°C | Oryctolagus cuniculus |