Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates, synergistic with EDTA | Cyprinus carpio | |
cysteine | activates, synergistic with EDTA | Cyprinus carpio | |
EDTA | activates | Cyprinus carpio | |
thiol compounds | activate | Cyprinus carpio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 4% inhibition at 5 mM | Cyprinus carpio | |
antipain | 91% inhibition at 0.1 mM | Cyprinus carpio | |
CdCl2 | 90% inhibition at 1 mM | Cyprinus carpio | |
CoCl2 | 100% inhibition at 1 mM | Cyprinus carpio | |
CuCl2 | 94% inhibition at 1 mM | Cyprinus carpio | |
DTNB | 100% inhibition at 1 mM | Cyprinus carpio | |
ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate | 100% inhibition at 0.1 mM | Cyprinus carpio | |
HgCl2 | 73% inhibition at 1 mM | Cyprinus carpio | |
leupeptin | 80% inhibition at 0.1 mM | Cyprinus carpio | |
PCMB | 46% inhibition at 1 mM | Cyprinus carpio | |
Soybean trypsin inhibitor | 8% inhibition at 0.005 mM | Cyprinus carpio | |
tosyl-Leu-CH2Cl | 89% inhibition at 1 mM | Cyprinus carpio | |
tosyl-Phe-CH2Cl | 84%inhibition at 1 mM | Cyprinus carpio | |
Zn(CH3COO)2 | 85% inhibition at 1 mM | Cyprinus carpio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
gel filtration | Cyprinus carpio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyprinus carpio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cyprinus carpio |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000109 | - |
- |
Cyprinus carpio |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Cyprinus carpio |
More | two bands in SDS-PAGE of 23000 and 27000, respectively, interpreted as different stages of enzyme processing | Cyprinus carpio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
substrate Arg-4-methylcoumaryl-7-amide | Cyprinus carpio |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
rapid inactivation above, without substrate bound | Cyprinus carpio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate Arg-4-methylcoumaryl-7-amide | Cyprinus carpio |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | rapid inactivation above | Cyprinus carpio |