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Literature summary for 3.4.22.16 extracted from
- Human cathepsin H (1980), Biochem. J., 191, 487-497.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminophenylmercuric acetate | 93% inhibition at 1 mM | Homo sapiens |  |
| Benzyloxycarbonyl-Phe-Phe-CHN2 | 31% inhibition at 0.01 mM | Homo sapiens | |
| chymostatin B | 47% inhibition at 0.016 mM | Homo sapiens | |
| Hyamine 1622 | 52% inhibition at 1 mM | Homo sapiens | |
| iodoacetamide | 99% inhibition at 1 mM | Homo sapiens | |
| iodoacetic acid | 99% inhibition at 1 mM | Homo sapiens | |
| iodoacetic acid | - |
Rattus norvegicus | |
| Leu-CH2Cl | 85% inhibition at 0.001 mM | Homo sapiens | |
| leupeptin | 29% inhibition at 0.021 mM | Homo sapiens | |
| leupeptin | - |
Rattus norvegicus | |
| N-ethylmaleimide | 69% inhibition at 1 mM | Homo sapiens | |
| puromycin | 36% inhibition at 1 mM | Homo sapiens | |
| Soybean trypsin inhibitor | non-specific | Homo sapiens | |
| tosyl-Leu-CH2Cl | 60% inhibition at 0.01 mM | Homo sapiens | |
| tosyl-Leu-CH2Cl | - |
Rattus norvegicus | |
| tosyl-Phe-CH2Cl | 79% inhibition at 0.01 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
| 0.15 | - |
Arg-4-methylcoumaryl-7-amide | - |
Homo sapiens | |
| 0.637 | - |
benzoyl-Arg-2-naphthylamide | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
- |
Homo sapiens |
| 28000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | - |
Homo sapiens |
| side-chain modification | - |
Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
- |
Rattus norvegicus |
| 0.15 | - |
substrate benzyloxycarbonyl-Arg-Arg-2-naphthylamide | Homo sapiens |
| 1.73 | - |
substrate benzoyl-DL-Arg-2-naphthylamide | Homo sapiens |
| 1.95 | - |
substrate Leu-2-naphthylamide | Homo sapiens |
| 3.5 | - |
substrate Arg-2-naphthylamide | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, 50-100 mM Na-acetate buffer, pH 5.5, 1 mM Na2-EDTA, 0.1% Brij 35, 5 mM Na-tetrathionate, 1 month, 0-10% loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O | - |
Homo sapiens | alpha-N-benzoyl-DL-arginine + beta-naphthylamine | - |
? | |
| Arg-2-naphthylamide + H2O | - |
Homo sapiens | Arg + 2-naphthylamine | - |
ir | |
| Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | Arg + 2-naphthylamine | - |
ir | |
| Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Homo sapiens | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
| benzoyl-DL-Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | benzoyl-DL-Arg + 2-naphthylamine | - |
ir | |
| Leu-2-naphthylamide + H2O | - |
Homo sapiens | Leu + 2-naphthylamine | - |
ir | |
| Leu-2-naphthylamide + H2O | - |
Rattus norvegicus | Leu + 2-naphthylamine | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
| monomer | - |
Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-N-benzoyl-arginine 2-naphthylamide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Homo sapiens |
| 40 | - |
- |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, 15% loss of activity | Homo sapiens |
| 60 | - |
30 min, 80% loss of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | substrate azocasein | Homo sapiens |
| 5 | 6 | substrate azocasein | Rattus norvegicus |
| 6.8 | - |
- |
Rattus norvegicus |
| 6.8 | - |
synthetic substrates | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | less than 20% of maximal activity above and below | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
irreversibly inactivated at alkaline pH | Homo sapiens |
| 7 | - |
irreversible inactivation above | Homo sapiens |
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Release 2023.1 (January 2023)
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