Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | native cathepsin L was completely inhibited by 0.001, 0.004, or 0.01 mM cathepsin L propeptide (10 min, pH 6.5, room temperature) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Renatured (Comment) | Organism |
---|---|
the optimum cathepsin L renaturation buffer consists of 50 mM Tris, 4 mM GSH, 0.5 mM GSSG, 300 mM MgSO4, 0.05% (w/v) 3-(3-cholamidopropyl)-dimethylammonio-1-propane sulfonate, 0.04 mM propetide, at pH 7.75 and 16°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? |