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Literature summary for 3.4.22.15 extracted from

  • Johnson, G.D.; Jiang, W.
    Characterization of cathepsin L secreted by Sf21 insect cells (2005), Arch. Biochem. Biophys., 444, 7-14.
    View publication on PubMed
Search for more literature for 3.4.22.15

Inhibitors

Inhibitors Comment Organism Structure
cystatin A
-
 Spodoptera frugiperda
cystatin B
-
 Spodoptera frugiperda
cystatin D
-
 Spodoptera frugiperda
cystatin E/M
-
 Spodoptera frugiperda
cystatin F
-
 Spodoptera frugiperda
cystatin SA
-
 Spodoptera frugiperda
cystatin SN
-
 Spodoptera frugiperda
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Spodoptera frugiperda
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 SDS-PAGE Spodoptera frugiperda
405000
-
 gel filtration Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein above 25% carbohydrate content Spodoptera frugiperda
proteolytic modification proteolytic activation occurs in two steps: cleavage of the 48 kDa proenzyme to a 34 kDa species and then to 32 kDa mature enzyme Spodoptera frugiperda

Purification (Commentary)

Purification (Comment) Organism
3 chromatography steps Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
SF-21 cell
-
 Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008
-
 tert-butoxycarbonyl-Val-Pro-Arg-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.01
-
 tert-butoxycarbonyl-Arg-Val-Arg-Arg-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.012
-
 succinyl-Leu-Leu-Val-Tyr-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.03
-
 tert-butoxycarbonyl-Arg-Arg-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.36
-
 Leu-Tyr-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.38
-
 succinly-Ala-Phe-Lys-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
3.9
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
8.7
-
 benzyloxycarbonyl-Leu-Arg-4-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
11
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide, 0484 nM enzyme, 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Arg-4-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Spodoptera frugiperda Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
succinyl-Ala-Phe-Lys-4-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Spodoptera frugiperda succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
tert-butoxycarbonyl-Arg-Arg-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda tert-butoxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butoxycarbonyl-Arg-Val-Arg-Arg-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda tert-butoxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
tert-butoxycarbonyl-Val-Pro-Arg-methylcoumarin 7-amide + H2O
-
 Spodoptera frugiperda tert-butoxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 48000, SDS-PAGE and gel filtration Spodoptera frugiperda

Synonyms

Synonyms Comment Organism
cathepsin L
-
 Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Spodoptera frugiperda

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5
-
 Spodoptera frugiperda

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000019
-
 cystatin E/M 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000021
-
 cystatin A 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000021
-
 cystatin F 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000027
-
 cystatin SN 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000046
-
 cystatin B 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000051
-
 cystatin SA 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.00000059
-
 trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda
0.0000065
-
 cystatin D 50 mM sodium acetate, 1 mM dithiothreitol, pH 5.0, 24°C Spodoptera frugiperda