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Literature summary for 3.4.22.15 extracted from
- Purification and some properties of a myofibrillar protein-degrading protease, cathepsin L, from rabbit skeletal muscle (1980), J. Biochem., 87, 1133-1143.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 0.3 mM, slight activation | Oryctolagus cuniculus |  |
| EDTA | essential for activity | Oryctolagus cuniculus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Oryctolagus cuniculus | |
| iodoacetamide | - |
Oryctolagus cuniculus | |
| iodoacetic acid | strong | Oryctolagus cuniculus | |
| L-1-tosyl-2-phenylethylchloromethylketone | - |
Oryctolagus cuniculus | |
| leupeptin | - |
Oryctolagus cuniculus | |
| additional information | no inhibition by pepstatin, phenylmethane sulfonyl fluoride | Oryctolagus cuniculus | |
| tosyl-Lys-CH2Cl | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | skeletal muscle myofibrillar enzyme | Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme removes the Ca-sensitivity of ATPase activity of myofibrils | Oryctolagus cuniculus | ? | - |
? | |
| myosin + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
pH 6.0, 10 min, 50% loss of activity | Oryctolagus cuniculus |
| 70 | - |
pH 6.0, 10 min, complete loss of activity | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
hydrolysis of myosin | Oryctolagus cuniculus |
| 7 | - |
removal of the Ca-sensitivity of ATPase activity of myofibrils | Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | 37°C, 1 h, stable | Oryctolagus cuniculus |
| 7.5 | - |
37°C, 1 h, unstable above | Oryctolagus cuniculus |
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