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Literature summary for 3.4.22.10 extracted from

  • Kortt, A.A.; Liu, T.Y.
    On the mechanism of action of streptococcal proteinase. II. Comparison of the kinetics of proteinase- and papain-catalyzed hydrolysis of N-acylamino acid esters (1973), Biochemistry, 12, 328-337.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effect of methanol of Km-value for N-benzyloxycarbonyl-L-Ala, p-nitrophenyl alpha-N-benzyloxycarbonyl-L-Lys and N-benzyloxycarbonyl-Gly Streptococcus sp.
0.0236
-
 N-benzyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.063
-
 p-nitrobenzyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.132
-
 N-benzyloxycarbonyl-Ala-p-nitrophenyl ester
-
 Streptococcus sp.
0.334
-
 cinnamoyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.422
-
 N-benzoyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.438
-
 t-butyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.61
-
 tert-butyloxycarbonyl-Ala-p-nitrophenyl ester
-
 Streptococcus sp.

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Ala + H2O
-
 Streptococcus sp. ?
-
 ?
benzyloxycarbonyl-Gly-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. ?
-
 ?
cinnamoyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. cinnamoyl-Gly + 4-nitrophenol
-
 ?
N-benzoyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. N-benzoyl-Gly + 4-nitrophenol
-
 ?
N-benzyloxycarbonyl-Ala-p-nitrophenyl ester + H2O
-
 Streptococcus sp. N-benzyloxycarbonyl-Ala + 4-nitrophenol
-
 ?
N-benzyloxycarbonyl-Gly + H2O
-
 Streptococcus sp. ?
-
 ?
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. N-benzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
p-nitrobenzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. p-nitrobenzyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
t-butyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
 Streptococcus sp. t-butyloxycarbonyl-Gly + 4-nitrophenol
-
 ?
tert-butyloxycarbonyl-Ala-p-nitrophenyl ester + H2O
-
 Streptococcus sp. tert-butyloxycarbonyl-Ala + 4-nitrophenol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.147
-
 t-butyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
0.21
-
 N-benzoyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
1.15
-
 p-nitrobenzyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
1.52
-
 cinnamoyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
4.31
-
 tert-butyloxycarbonyl-Ala-p-nitrophenyl ester
-
 Streptococcus sp.
9.4
-
 N-benzyloxycarbonyl-Gly-p-nitrophenyl ester
-
 Streptococcus sp.
118
-
 N-benzyloxycarbonyl-Ala-p-nitrophenyl ester
-
 Streptococcus sp.