Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | reduction is essential for catalytic activity | Streptococcus sp. | |
thioethanol | reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity | Streptococcus sp. | |
thioglycolic acid | reduction is essential for catalytic activity | Streptococcus sp. |
Crystallization (Comment) | Organism |
---|---|
- |
Streptococcus sp. |
General Stability | Organism |
---|---|
0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation | Streptococcus sp. |
0.1 N HCl, 2 h, denaturation of the zymogen | Streptococcus sp. |
0.5% SDS, denaturation | Streptococcus sp. |
8 M urea plus reducing agent, 20% of proteolytic activity is provided | Streptococcus sp. |
EDTA, 0.001 M, stabilizes | Streptococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrofluorobenzene | - |
Streptococcus sp. | |
Benzyloxycarbonyl-Gly-Phe | competitive against benzyloxycarbonyl-Phe-Tyr | Streptococcus sp. | |
iodoacetamide | - |
Streptococcus sp. | |
iodoacetic acid | - |
Streptococcus sp. | |
methyliodine | - |
Streptococcus sp. | |
NEM | - |
Streptococcus sp. | |
Sodium tetrathionate | - |
Streptococcus sp. | |
Tosyl-norleucine-Phe | competitive against benzyloxycarbonyl-norleucine-Phe | Streptococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of pH-value on the Km-value for benzyloxycarbonyl-Lys-p-nitrophenyl ester | Streptococcus sp. | |
0.22 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.27 | Streptococcus sp. | |
0.262 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 6.35 | Streptococcus sp. | |
0.494 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.49 | Streptococcus sp. | |
2.28 | - |
benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
2.64 | - |
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
3.88 | - |
benzyloxycarbonyl-Glu-Phe | - |
Streptococcus sp. | |
5.05 | - |
Nalpha-benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
bisulfite | reduction is essential for catalytic activity | Streptococcus sp. | |
cyanide | reduction is essential for catalytic activity | Streptococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
high-speed equilibrium ultracentrifugation | Streptococcus sp. |
33590 | - |
determination of amino acid composition | Streptococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sp. | - |
hemolytic | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Streptococcus sp. |
Renatured (Comment) | Organism |
---|---|
90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold | Streptococcus sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 years | Streptococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | ? | - |
? | |
benzyloxycarbonyl-Glu-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | benzyloxycarbonyl-Gly + p-nitrophenol | - |
? | |
benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | ? | - |
? | |
benzyloxycarbonyl-Nle-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | N-benzyloxycarbonyl-Gly + 4-nitrophenol | - |
? | |
Nalpha-benzyloxycarbonyl-Lys-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O | - |
Streptococcus sp. | ? | - |
? | |
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol | - |
? | |
Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O | - |
Streptococcus sp. | ? | - |
? | |
Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O | - |
Streptococcus sp. | Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
0.1 N HCl, 2 h, denaturation of the zymogen | Streptococcus sp. |
60 | - |
irreversible inactivation | Streptococcus sp. |
100 | - |
pH 6.7, 10 min, denaturation of the zymogen | Streptococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of pH-value on the turnover numer for benzyloxycarbonyl-Lys-p-nitrophenyl ester | Streptococcus sp. | |
1.09 | - |
benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
2.3 | - |
benzyloxycarbonyl-Glu-Phe | - |
Streptococcus sp. | |
5.05 | - |
Nalpha-benzyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
8.6 | - |
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe | - |
Streptococcus sp. | |
29.7 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.27 | Streptococcus sp. | |
89.8 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 4.49 | Streptococcus sp. | |
142 | - |
benzyloxycarbonyl-Lys-p-nitrophenyl ester | pH 6.35 | Streptococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | unstable below pH 4.5 and above pH 9.5 | Streptococcus sp. |