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Literature summary for 3.4.22.10 extracted from

  • Tsai, W.H.; Chang, C.W.; Chuang, W.J.; Lin, Y.S.; Wu, J.J.; Liu, C.C.; Chang, W.T.; Lin, M.T.
    Streptococcal pyrogenic exotoxin B-induced apoptosis in a549 cells is mediated by a receptor- and mitochondrion-dependent pathway (2004), Infect. Immun., 72, 7055-7062.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and inactive mutant enzyme in Escherichia coli strain BL21(DE3) and in A-549 cells Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
C192S inactive mutant Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E64, complete inhibition at 0.016 mM Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 42000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes
42000
-
 x * 42000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pyogenes the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview ?
-
 ?
additional information Streptococcus pyogenes A-20 the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes A-20
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme performs autolytic processing to the mature 28 kDa protein by removal of an about 117 amino-acid peptide from the N-terminus Streptococcus pyogenes

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and inactive mutant enzyme from Escherichia coli by affinity chromatography Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview Streptococcus pyogenes ?
-
 ?
additional information the enzyme performs autolytic activity and also cleaves the inactive mutant C192S Streptococcus pyogenes ?
-
 ?
additional information the enzyme can induce apoptosis in A549 cells, the induction of apoptosis in cells requires the protease activity and the propper enzyme size of 28 kDa, cell binding activity of processed and unprocessed wild-type and mutant enzymes, the induction can be prevented by inhibition of caspase-8, induction cascade, overview Streptococcus pyogenes A-20 ?
-
 ?
additional information the enzyme performs autolytic activity and also cleaves the inactive mutant C192S Streptococcus pyogenes A-20 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
Spe B
-
 Streptococcus pyogenes
SPE B protease
-
 Streptococcus pyogenes
streptococcal pyrogenic exotoxin B
-
 Streptococcus pyogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 10 min, inactivation Streptococcus pyogenes