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Literature summary for 3.4.22.1 extracted from

  • Chen, W.; Ning, D.; Wang, X.; Chen, T.; Lv, X.; Sun, J.; Wu, D.; Huang, Y.; Xu, J.; Yu, X.
    Identification and characterization of Clonorchis sinensis cathepsin B proteases in the pathogenesis of clonorchiasis (2015), Parasit. Vectors, 8, 647 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Clonorchis sinensis

Inhibitors

Inhibitors Comment Organism Structure
AEBSF 0.2 mM, partial inhibition; 0.2 mM, partial inhibition; 0.2 mM, partial inhibition; 0.2 mM, partial inhibition Clonorchis sinensis
Ca-074 methyl ester 0.001 mM, complete inhibition; 0.001 mM, complete inhibition; 0.001 mM, complete inhibition; 0.001 mM, complete inhibition Clonorchis sinensis
E-64 0.02 mM, complete inhibition; 0.02 mM, complete inhibition; 0.02 mM, complete inhibition; 0.02 mM, complete inhibition Clonorchis sinensis
iodoacetic acid 0.01 mM, partial inhibition; 0.01 mM, partial inhibition; 0.01 mM, partial inhibition; 0.01 mM, partial inhibition Clonorchis sinensis
PMSF 2 mM, partial inhibition; 2 mM, partial inhibition; 2 mM, partial inhibition; 2 mM, partial inhibition Clonorchis sinensis
TPCK 0.2 mM, partial inhibition; 0.2 mM, partial inhibition; 0.2 mM, partial inhibition; 0.2 mM, partial inhibition Clonorchis sinensis

Organism

Organism UniProt Comment Textmining
Clonorchis sinensis
-
-
-
Clonorchis sinensis A1YLF1
-
-
Clonorchis sinensis A1YLF2
-
-
Clonorchis sinensis B0L0Y4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clonorchis sinensis

Source Tissue

Source Tissue Comment Organism Textmining
excretory canal cell in metacercaria and cercaria, four cathepsin Bs can be detected in the excretory vesicle and oral sucker Clonorchis sinensis
-
gastrointestinal tract in adult worm, four cathepsin Bs can be specifically observed in the intestinal tract Clonorchis sinensis
-
oral sucker in metacercaria and cercaria, four cathepsin Bs can be detected in the excretory vesicle and oral sucker Clonorchis sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Clonorchis sinensis benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O low activity with cathepsin B3 and B2, higher activity with cathepsin B1 and B4 Clonorchis sinensis benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
human fibronectin + H2O
-
 Clonorchis sinensis ?
-
 ?
human hemoglobin + H2O
-
 Clonorchis sinensis ?
-
 ?
human IgG + H2O
-
 Clonorchis sinensis ?
-
 ?
human serum albumin + H2O
-
 Clonorchis sinensis ?
-
 ?

Synonyms

Synonyms Comment Organism
CsCB1
-
 Clonorchis sinensis
CsCB2
-
 Clonorchis sinensis
CsCB3
-
 Clonorchis sinensis
CsCB4
-
 Clonorchis sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B2 Clonorchis sinensis
37 42 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B3 Clonorchis sinensis
42
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B1 Clonorchis sinensis
42
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B4 Clonorchis sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B4 Clonorchis sinensis
5
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B1 Clonorchis sinensis
5.5
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B2 Clonorchis sinensis
5.5
-
 substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B3 Clonorchis sinensis

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 pH 4.0: about 50% of maximal activity, pH 6.0: about 70% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B2 Clonorchis sinensis
4 6.5 pH 4.0: about 55% of maximal activity, pH 6.0: about 80% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B3 Clonorchis sinensis
4 6.5 pH 4.0: about 60% of maximal activity, pH 6.0: about 50% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B4 Clonorchis sinensis
4 6.5 pH 4.0: about 70% of maximal activity, pH 6.0: about 80% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, cathepsin B1 Clonorchis sinensis

General Information

General Information Comment Organism
additional information secreted cathepsin Bs are involved in the pathogenesis of clonorchiasis Clonorchis sinensis