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Literature summary for 3.4.22.1 extracted from

  • Ranjit, N.; Zhan, B.; Stenzel, D.J.; Mulvenna, J.; Fujiwara, R.; Hotez, P.J.; Loukas, A.
    A family of cathepsin B cysteine proteases expressed in the gut of the human hookworm, Necator americanus (2008), Mol. Biochem. Parasitol., 160, 90-99.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-2 in insoluble inclusion bodies in Escherichia coli Necator americanus
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-3 in soluble, secreted form in Pichia pastoris strain X-33 Necator americanus
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-4 in insoluble inclusion bodies in Escherichia coli Necator americanus
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-5 in insoluble inclusion bodies in Escherichia coli Necator americanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE Necator americanus
55000
-
x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE Necator americanus

Organism

Organism UniProt Comment Textmining
Necator americanus
-
cathepsin B-like cysteine protease Na-CP-2
-
Necator americanus
-
cathepsin B-like cysteine protease Na-CP-3
-
Necator americanus
-
cathepsin B-like cysteine protease Na-CP-4
-
Necator americanus
-
cathepsin B-like cysteine protease Na-CP-5
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Na-CP-2 N-glycosylation site at position 140 Necator americanus
glycoprotein Na-CP-3 N-glycosylation site at positions 32, 136, 242, and 298 Necator americanus
glycoprotein Na-CP-4 N-glycosylation site at positions 134 and 296 Necator americanus
glycoprotein Na-CP-5 N-glycosylation site at positions 101, 135 and 245 Necator americanus
proteolytic modification Na-CP-3 autoactivates by proteolytic cleavage at pH 4.0 Necator americanus

Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme Na-CP-2 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions Necator americanus
recombinant isozyme Na-CP-4 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions Necator americanus
recombinant isozyme Na-CP-5 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions Necator americanus
recombinant Na-CP-3 from yeast cell culture supernatant by nickel affinity chromatography Necator americanus

Source Tissue

Source Tissue Comment Organism Textmining
gut Na-CP-2 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm Necator americanus
-
gut Na-CP-3 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm Necator americanus
-
gut Na-CP-4 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm Necator americanus
-
gut Na-CP-5 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm Necator americanus
-
additional information developmental expression pattern, the isozyme is highly up-regulated in the adult stage, overview Necator americanus
-
additional information developmental expression patterns, the isozymes are highly up-regulated in the adult stage, overview Necator americanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O a fluorogenic substrate of Na-CP-3 Necator americanus carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin
-
?
Gelatin + H2O a substrate of Na-CP-3 Necator americanus ?
-
?
additional information no activity with carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin by Na-CP-3, recombinant Na-CP-3 does not digest intact hemoglobin but digests globin fragments generated by prior hydrolysis with aspartic hemoglobinases Necator americanus ?
-
?

Subunits

Subunits Comment Organism
? x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE Necator americanus

Synonyms

Synonyms Comment Organism
CatB
-
Necator americanus
cathepsin B-like cysteine protease
-
Necator americanus
Na-CP-2
-
Necator americanus
Na-CP-3
-
Necator americanus
Na-CP-4
-
Necator americanus
Na-CP-5
-
Necator americanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 recombinant isozyme Na-CP-3 Necator americanus

pH Range

pH Minimum pH Maximum Comment Organism
4 7 recombinant isozyme Na-CP-3 Necator americanus