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Literature summary for 3.4.22.1 extracted from

  • Yakovlev, A.A.; Gorokhovatsky, A.Y.; Onufriev, M.V.; Beletsky, I.P.; Gulyaeva, N.V.
    Brain cathepsin B cleaves a caspase substrate (2008), Biochemistry (Moscow), 73, 332-336.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the presence of dithiothreitol at concentrations of more than 10 mM significantly increases activity at pH 4.0 Rattus norvegicus
reduced glutathione the presence of reduced glutathione at concentrations of more than 10 mM significantly increases activity at pH 4.0 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Asp-Glu-Val-Asp-CHO complete DEVDase activity inhibition in brain cell supernatant Rattus norvegicus
acetyl-DEVD-CHO
-
Rattus norvegicus
Ca2+ 40% inhibition at 2 mM Rattus norvegicus
iodoacetamide concentrations above10 mM significantly decrease activity Rattus norvegicus
Mg2+ 50% inhibition at 2 mM Rattus norvegicus
Mn2+ 10% inhibition at 8 mM, no inhibition at 2 mM Rattus norvegicus
N-ethylmaleimide concentrations above10 mM significantly decrease activity Rattus norvegicus
oxidized glutathione concentrations above10 mM significantly decrease activity Rattus norvegicus
Zn2+ 80% inhibition at 2 mM Rattus norvegicus
[L-3-trans-(propylcarbamoyl)oxirane-2-carbonyl]-L-isoleucyl-L-proline
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates Rattus norvegicus 5737
-
lysosome
-
Rattus norvegicus 5764
-
lysosome in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates Rattus norvegicus 5764
-
soluble
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from brain cell supernatant by ammonium sulfate fractionation and gel filtration Rattus norvegicus
S-Sepharose Fast Flow column chromatography and Sephadex G-25 gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
neuroblastoma cell
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O a synthetic substrate of caspase-3 and caspase-7 Rattus norvegicus ?
-
?
acetyl-DEVD-7-amido-4-methylcoumarin + H2O cleavage occurs at pH 4.0, but not at pH 7.5 Rattus norvegicus acetyl-DEVD + 7-amino-4-methylcoumarin
-
?
additional information under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
CatB
-
Rattus norvegicus
DEVDase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Rattus norvegicus
4
-
with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5 activity range with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, about 90% of maximal activity at pH 3.5, maximal activity at pH 4.0, and about 5-10% of maximal activity at pH 6.5, iactive at pH 7.0 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
no activity at pH 7.5 Rattus norvegicus