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Literature summary for 3.4.21.B55 extracted from
- Characterization of pyrolysin, a hyperthermoactive serine protease from the archaebacterium Pyrococcus furiosus (1990), FEMS Microbiol. Lett., 71, 17-20.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphate | 0.5 mM, complete inhibition | Pyrococcus furiosus |  |
| EDTA | 10 mM, 27% inhibition | Pyrococcus furiosus | |
| additional information | no inhibition by cysteine-type protease inhibitors pCMB and iodoacetate | Pyrococcus furiosus | |
| PMSF | 1 mM, 87% inhibition | Pyrococcus furiosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell envelope | 75% of the activity is associated with the cell-envelope | Pyrococcus furiosus | 30313 | - |
| cytoplasm | 25% of the activity was located in the cytoplasm | Pyrococcus furiosus | 5737 | - |
| additional information | no activity is observed in the medium when Pyrococcus furiosus is grown in batch culture | Pyrococcus furiosus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | P72186 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-stable enzyme | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 115 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
t1/2 is above 6000 min | Pyrococcus furiosus |
| 95 | - |
t1/2: 540 min | Pyrococcus furiosus |
| 100 | - |
t1/2: 240 min | Pyrococcus furiosus |
| 105 | - |
t1/2: 20 min | Pyrococcus furiosus |
| 110 | - |
t1/2: 3 min | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | broad pH range with at least 50% of its maximal activity between pH 6.5 and 10.5 | Pyrococcus furiosus |
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