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Literature summary for 3.4.21.B55 extracted from

  • Dai, Z.; Fu, H.; Zhang, Y.; Zeng, J.; Tang, B.; Tang, X.F.
    Insights into the maturation of hyperthermophilic pyrolysin and the roles of its N-terminal propeptide and long C-terminal extension (2012), Appl. Environ. Microbiol., 78, 4233-4241.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B55

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant proform of Pyrococcus furiosus pyrolysin and several N- and C-terminal deletion mutants are successfully expressed in Escherichia coli Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.32
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus
3.83
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P72186
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the maturation of the enzyme involves autoprocessing of both the N-propeptide and a C-terminal propeptide. The long C-terminal extension can be dissected into a C-terminal propeptide and a C-terminal extension (CTEm) that is attached to the catalytic domain of the mature enzyme. Both of these propeptides assist in achieving pyrolysin hyperthermostability Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Pyrococcus furiosus ?
-
 ?
succinyl-Ala-Ala-Pro-Lys-4-nitroanilide + H2O
-
 Pyrococcus furiosus succinyl-Ala-Ala-Pro-Lys + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1893
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus
3128
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
494
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme lacking the C-terminal extension (mPlsDELTAC740) Pyrococcus furiosus
942
-
 succinyl-Ala-Ala-Pro-Lys-4-nitroanilide pH 7.5, 90°C, mature enzyme Pyrococcus furiosus