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Literature summary for 3.4.21.B45 extracted from

  • Ramachandran, R.; Eissa, A.; Mihara, K.; Oikonomopoulou, K.; Saifeddine, M.; Renaux, B.; Diamandis, E.; Hollenberg, M.D.
    Proteinase-activated receptors (PARs): differential signalling by kallikrein-related peptidases KLK8 and KLK14 (2012), Biol. Chem., 393, 421-427.
    View publication on PubMed
Search for more literature for 3.4.21.B45

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme activates the proteinase-activated receptor 2 recombinnatly expressed in rat and in human cells ?
-
 ?
proteinase-activated receptor 2 zymogen + H2O Homo sapiens
-
 mature proteinase-activated receptor 2 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9P0G3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDDSTPSILPAPRGYPGQV + H2O the tethered ligand is GYPGQV Homo sapiens GDDSTPSILPAPR + GYPGQV
-
 ?
GPNSKGRSLIGRLDTPYGGC + H2O the tethered ligand is SLIGRL Homo sapiens SLIGRLDTPYGGC + RLDTPYGGC + GPNSKGRSLIG
-
 ?
GTNRSSKGRSLIGKVDGTSHVTGKGVT + H2O the tethered ligand is SLIGKV Homo sapiens GTNRSSKGR + SLIGKVDGTSHVTGKGVT
-
 ?
human proteinase-activated receptor 2 zymogen + H2O unmasking the PAR2 receptor-activating sequence from a peptide precursor Homo sapiens mature human proteinase-activated receptor 2 + ?
-
 ?
additional information the enzyme activates the proteinase-activated receptor 2 recombinnatly expressed in rat and in human cells Homo sapiens ?
-
 ?
additional information KLK14 can cleave synthetic peptides representing the cleavage-activation sequences of human proteinase-activated receptors PARs 1, 2 and 4 to unmask a receptor-activating sequence. Substrate specificity, overview. Analysis of processing of synthetic human and rat proteinase-activated receptor 2, PAR2, derived sequences, representing the cleavage activation domain of PAR2, by kallikrein 8 versus kallikrein 14. KLKs 8 and 14 can both cleave the synthetic PAR2 tethered ligand-containing synthetic peptides to unmask a potential receptor-activating sequence, but each KLK exhibits distinct signalling properties via PARs 1 and 2 Homo sapiens ?
-
 ?
NATLDPRSFLLRNPNDKYE + H2O the tethered ligand is SFLLRN Homo sapiens NATLDPR + SFLLRNPNDKYE
-
 ?
proteinase-activated receptor 2 zymogen + H2O
-
 Homo sapiens mature proteinase-activated receptor 2 + ?
-
 ?
rat proteinase-activated receptor 2 zymogen + H2O unmasking the PAR2 receptor-activating sequence from a peptide precursor Homo sapiens mature rat proteinase-activated receptor 2 + ?
-
 ?

Synonyms

Synonyms Comment Organism
kallikrein-related peptidase 14
-
 Homo sapiens
KLK14
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism both KLK14 and trypsin are able to activate MAP kinase signalling in the human HEK cells as does the PAR-activating peptide, while KLK8 does not Homo sapiens
physiological function human enzyme can activate rat and human proteinase-activated receptor 2, PAR2, calcium signalling, and the enzyme stimulates human PAR2 clustering and internalization Homo sapiens