BRENDA - Enzyme Database show
show all sequences of 3.4.21.B42

A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate

Mitsui, S.; Yamada, T.; Okui, A.; Kominami, K.; Uemura, H.; Yamaguchi, N.; Biochem. Biophys. Res. Commun. 272, 205-211 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection; gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
secretion into the medium
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Homo sapiens
44000
-
x * 44000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
polypeptide + H2O
Homo sapiens
-
peptides
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9UBX7
brain-type isozyme; prostate-type isozyme; splicing variant 1; splicing variant 2
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
4 possible glycosylation sites
Homo sapiens
Reaction
Reaction
Commentary
Organism
proteolytic cleavage of polypeptides, preferential cleavage site: Arg-
enzyme is trypsin-like a serine protease
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
brain-type isozyme, secreted into the medium
Homo sapiens
-
epithelium
prostate
Homo sapiens
-
heart
brain-type isozyme
Homo sapiens
-
hippocampus
especially expressed in pyramidal neurons
Homo sapiens
-
lung
prostate-type isozyme
Homo sapiens
-
neuron
pyramidal, hippocampus
Homo sapiens
-
prostate
especially expressed in secretory epithelial cells; prostate-type isozyme, secreted into the medium
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
acetyl-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
best substrate
647365
Homo sapiens
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyl-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyl-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
polypeptide + H2O
-
647365
Homo sapiens
peptides
-
647365
Homo sapiens
?
Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Pyr-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE; x * 44000, SDS-PAGE
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection; gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
secretion into the medium
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, SDS-PAGE
Homo sapiens
44000
-
x * 44000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
polypeptide + H2O
Homo sapiens
-
peptides
-
Homo sapiens
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
4 possible glycosylation sites
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
brain-type isozyme, secreted into the medium
Homo sapiens
-
epithelium
prostate
Homo sapiens
-
heart
brain-type isozyme
Homo sapiens
-
hippocampus
especially expressed in pyramidal neurons
Homo sapiens
-
lung
prostate-type isozyme
Homo sapiens
-
neuron
pyramidal, hippocampus
Homo sapiens
-
prostate
especially expressed in secretory epithelial cells; prostate-type isozyme, secreted into the medium
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
acetyl-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
best substrate
647365
Homo sapiens
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyl-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyl-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
polypeptide + H2O
-
647365
Homo sapiens
peptides
-
647365
Homo sapiens
?
Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O
recombinant enzyme
647365
Homo sapiens
Pyr-Gly-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE; x * 44000, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Other publictions for EC 3.4.21.B42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732964
Xu
The diagnostic and prognostic ...
Homo sapiens
Tumour Biol.
35
5199-5203
2014
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718041
Wen
Identification and validation ...
Homo sapiens
J. Surg. Oncol.
104
516-524
2011
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1
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2
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1
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1
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718165
Jamaspishvili
Immunohistochemical localizati ...
Homo sapiens
Neoplasma
58
298-303
2011
-
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4
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1
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1
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-
693983
Yu
Over-expression of human kalli ...
Homo sapiens
Med. Oncol.
27
40-44
2010
-
1
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2
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2
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1
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2
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709810
Bi
Association of TMPRSS2 and KLK ...
Homo sapiens
Med. Oncol.
27
145-151
2010
-
1
-
-
-
-
-
-
-
-
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2
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3
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1
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3
-
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-
-
-
-
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-
2
-
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2
-
-
691150
Shaw
Regulation of human tissue kal ...
Homo sapiens
Biol. Chem.
389
1409-1419
2008
-
-
-
-
-
-
-
-
-
-
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-
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1
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2
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2
-
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-
679272
McIntosh
Validation and characterizatio ...
Homo sapiens
Clin. Cancer Res.
13
4422-4428
2007
-
1
-
-
-
-
-
-
-
-
-
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2
-
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3
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1
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3
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680247
Sano
Kallikrein 11 expressed in hum ...
Homo sapiens
Int. J. Oncol.
30
1493-1498
2007
-
-
-
-
-
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1
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2
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7
-
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2
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1
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7
-
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2
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667873
Petraki
Cellular distribution of human ...
Homo sapiens
Biol. Chem.
387
653-663
2006
-
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1
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32
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32
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667878
Planque
Expression of the human kallik ...
Homo sapiens
Biol. Chem.
387
783-788
2006
-
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3
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667879
Scorilas
mRNA expression analysis of hu ...
Homo sapiens
Biol. Chem.
387
789-793
2006
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1
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1
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668284
Luo
Purification and characterizat ...
Homo sapiens
Clin. Cancer Res.
12
742-750
2006
-
-
-
-
-
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3
5
-
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1
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2
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1
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4
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5
1
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5
1
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5
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1
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1
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4
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5
1
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5
1
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668302
Sasaki
Decreased kallikrein 11 messen ...
Homo sapiens
Clin. Lung Cancer
8
45-48
2006
-
-
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3
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678527
Stephan
Improved prostate cancer detec ...
Homo sapiens
Biol. Chem.
387
801-805
2006
-
-
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3
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679747
Mitsui
Multiple promoters regulate ti ...
Homo sapiens
FEBS J.
273
3678-3686
2006
-
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1
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1
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4
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