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Literature summary for 3.4.21.B4 extracted from

  • Wilharm, E.; Parry, M.A.; Friebel, R.; Tschesche, H.; Matschiner, G.; Sommerhoff, C.P.; Jenne, D.E.
    Generation of catalytically active granzyme K from Escherichia coli inclusion bodies and identification of efficient granzyme K inhibitors in human plasma (1999), J. Biol. Chem., 274, 27331-27337.
    View publication on PubMed
Search for more literature for 3.4.21.B4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin 93% inactivation at 0.015 mM Homo sapiens
benzamidine 56% inactivation at 27 mM Homo sapiens
bikunin subunit of inter-alpha-trypsin inhibitor complex, physiologic inhibitor Homo sapiens
human blood plasma
-
 Homo sapiens
inter-alpha-trypsin inhibitor complex physiologic inhibitor Homo sapiens
Kunitz-type inhibitor aprotinin
-
 Homo sapiens
leupeptin 22% inactivation at 0.2 mM Homo sapiens
PefablocSC 94% inactivation at 1 mM Homo sapiens
Phe-Pro-Arg-chloromethyl ketone complete inactivation at 0.1 mM Homo sapiens
phenylmethylsulfonyl fluoride complete inactivation at 2 mM Homo sapiens
second carboxy-terminal Kunitz-type domain of bikunin physiologic inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 Nalpha-benzyloxycarbonyl-L-Arg-thiobenzyl ester pH 7.6 Homo sapiens
1.7
-
 Nalpha-benzyloxycarbonyl-L-Lys-thiobenzyl ester pH 7.6 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleaves synthetic thiobenzyl ester substrates after Lys and Arg, no substrates for the mature enzyme: Boc-Ala-Ala-Met-thiobenzyl ester and Boc-Ala-Ala-Asp-thiobenzyl ester Homo sapiens ?
-
 ?
Nalpha-benzyloxycarbonyl-L-Arg-thiobenzyl ester
-
 Homo sapiens ?
-
 ?
Nalpha-benzyloxycarbonyl-L-Lys-thiobenzyl ester
-
 Homo sapiens ?
-
 ?
Protein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
granzyme 3
-
 Homo sapiens
lymphocyte tryptase 2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.6
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000022
-
 second carboxy-terminal Kunitz-type domain of bikunin pH 7.6 Homo sapiens
0.00005
-
 bikunin pH 7.6 Homo sapiens
0.000064
-
 inter-alpha-trypsin inhibitor complex pH 7.6 Homo sapiens