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Literature summary for 3.4.21.B4 extracted from

  • Babe, L.M.; Yoast, S.; Dreyer, M.; Schmidt, B.F.
    Heterologous expression of human granzyme K in Bacillus subtilis and characterization of its hydrolytic activity in vitro (1998), Biotechnol. Appl. Biochem., 27, 117-124.
    View publication on PubMed
Search for more literature for 3.4.21.B4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis Homo sapiens

General Stability

General Stability Organism
enzyme quickly loses catalytic activity when diluted and stored in solution, Tween-20 or bovine serum albumin prevent loss of activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin complete inactivation at 0.1 mM Homo sapiens
benzamidine 50% inhibition at 27 mM Homo sapiens
leupeptin 50% inhibition at 0.2 mM Homo sapiens
phenylmethylsulfonyl fluoride complete inactivation at 2 mM Homo sapiens
Soybean trypsin inhibitor 50% inhibition at 0.025 mM Homo sapiens
Zn2+ significant reduction of enzyme activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
T-lymphocyte cytotoxic Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cys-Gly-Tyr-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Gly-Gly + H2O
-
 Homo sapiens ?
-
 ?
additional information for single-residue thioester substrates the enzyme shows a 2fold preference for a Lys versus Arg residue at P1. With oligopeptide substrates the enzyme displays peptidolytic activity C-terminal to both Lys and Arg residues with comparable rates of hydrolysis Homo sapiens ?
-
 ?
Protein + H2O
-
 Homo sapiens ?
-
 ?
Z-Lys-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens calculated from nucleotide sequence
-
 10.2