Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.B39 extracted from

  • Rajapakse, S.; Ogiwara, K.; Yamano, N.; Kimura, A.; Hirata, K.; Takahashi, S.; Takahashi, T.
    Characterization of mouse tissue kallikrein 5 (2006), Zool. Sci., 23, 963-968.
    View publication on PubMed
Search for more literature for 3.4.21.B39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
antipain 13% inhibition at 0.2 mM Mus musculus
Aprotinin 34% inhibition at 0.1 g/ml Mus musculus
diisopropylfluorophosphate 87% inhibition at 2 mM Mus musculus
E-64 12% inhibition at 0.5 mM Mus musculus
leupeptin 70% inhibition at 0.1 mM Mus musculus
additional information not inhibited by soybean trypsin inhibitor, benzamidine, pepstatin, EDTA, and chymostatin Mus musculus
N-alpha-p-tosyl-L-leucine-chloromethylketone 14% inhibition at 0.2 mM Mus musculus
N-alpha-p-tosyl-L-phenylalanine-chloromethylketone 14% inhibition at 0.2 mM Mus musculus
o-phenanthroline 10% inhibition at 2 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 diglutamyl-Gly-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.03
-
 diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.05
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.11
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.13
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 non-reducing SDS-PAGE Mus musculus
28500
-
 reducing SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelate column chromatography and Resource Q column chromatography Mus musculus

Storage Stability

Storage Stability Organism
-20°C, 2 months, no loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
collagen type IV + H2O
-
 Mus musculus ?
-
 ?
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Mus musculus diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Fibronectin + H2O
-
 Mus musculus ?
-
 ?
Gelatin + H2O
-
 Mus musculus ?
-
 ?
high-molecular-weight kininogen + H2O
-
 Mus musculus ?
-
 ?
insulin-like growth factor-binding protein-3 + H2O
-
 Mus musculus ?
-
 ?
additional information no activity with tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, casein, laminin, and single-chain tissue-type plasminogen activator Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
tissue kallikrein 5 serine protease with trypsin-like activity for Arg at the P1 position Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 diglutamyl-Gly-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.03
-
 diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.05
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.11
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
0.13
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
 Mus musculus