BRENDA - Enzyme Database show
show all sequences of 3.4.21.B30

Inhibition of RecA-mediated cleavage in covalent dimers of UmuD

Lee, M.H.; Guzzo, A.; Walker, G.C.; J. Bacteriol. 178, 7304-7307 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
V34C
defective for RecA-mediated cleavage
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
inactive UmuD is posttranslationally activated by a RecA-mediated cleavage at its Gys24-Gly25 bond that yields UmuD', UmuD monomer is a better substrate for the cleavage reaction than the dimer
Escherichia coli
Subunits
Subunits
Commentary
Organism
dimer
enzyme can form homodimers, crosslinking experiments, SDS-PAGE
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
V34C
defective for RecA-mediated cleavage
Escherichia coli
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
inactive UmuD is posttranslationally activated by a RecA-mediated cleavage at its Gys24-Gly25 bond that yields UmuD', UmuD monomer is a better substrate for the cleavage reaction than the dimer
Escherichia coli
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
enzyme can form homodimers, crosslinking experiments, SDS-PAGE
Escherichia coli
Other publictions for EC 3.4.21.B30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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9
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3
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1
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2
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731823
Hare
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FEMS Microbiol. Lett.
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2
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717851
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286
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718102
Ollivierre
Electron spin labeling reveals ...
Escherichia coli
Mol. Biosyst.
7
3183-3186
2011
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709122
Foti
UmuD2 inhibits a non-covalent ...
Escherichia coli
J. Biol. Chem.
285
23086-23095
2010
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709543
Fang
Conformational dynamics of the ...
Escherichia coli
J. Mol. Biol.
398
40-53
2010
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718023
Ollivierre
The roles of UmuD in regulatin ...
Escherichia coli
J. Nucleic Acids
2010
pii: 947680
2010
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1
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705887
Jiang
The active form of DNA polymer ...
Escherichia coli
Nature
460
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2009
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682581
Simon
Regulation of Escherichia coli ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
105
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2008
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681885
Godoy
UmuD and RecA directly modulat ...
Escherichia coli, Escherichia coli FC40
Mol. Cell
28
1058-1070
2007
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2
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669529
Beuning Penny
A non-cleavable UmuD variant t ...
Escherichia coli
J. Biol. Chem.
281
9633-9640
2006
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1
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4
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677665
Hare
A constitutively expressed, tr ...
Acinetobacter baylyi, Acinetobacter baylyi ADP1
Appl. Environ. Microbiol.
72
4036-4043
2006
-
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1
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11
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668385
Bridges
Error-prone repair and transle ...
Escherichia coli
DNA Repair
4
1047-1059
2005
1
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668384
Duzen Jill
Identification of specific ami ...
Escherichia coli
DNA Repair
3
301-312
2004
1
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668779
Paetzel
-
UmuD and UmuD proteins ...
Escherichia coli, Pseudomonas syringae, Salmonella enterica subsp. enterica serovar Typhimurium
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1976-1981
2004
1
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668783
Paetzel
-
UmuD and UmuD proteins ...
Escherichia coli
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner; J. F. , eds. )Academic Press
2
1976-1981
2004
1
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653588
Neher
Distinct peptide signals in th ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
100
13219-13224
2003
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1
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2
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651685
Sutton
Genetic and biochemical charac ...
Escherichia coli
J. Bacteriol.
183
347-357
2001
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1
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1
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650976
Gonzalez
Subunit-specific degradation o ...
Escherichia coli
EMBO J.
19
5251-5258
2000
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2
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651650
Mustard
Analysis of Escherichia coli R ...
Escherichia coli
J. Bacteriol.
182
1659-1670
2000
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653620
Sutton
The Escherichia coli SOS mutag ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
96
12373-12378
1999
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1
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651420
Gonzalez
Lon-mediated proteolysis of th ...
Escherichia coli
Genes Dev.
12
3889-3899
1998
-
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1
1
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1
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2
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1
1
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1
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1
1
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1
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1
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652778
Konola
Differential cleavage of LexA ...
Escherichia coli
J. Mol. Biol.
276
405-415
1998
-
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652782
McDonald
Regulation of UmuD cleavage: r ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
J. Mol. Biol.
282
721-730
1998
-
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1
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7
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4
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2
1
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1
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7
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2
1
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-
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-
-
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651628
Lee
Interactions of Escherichia co ...
Escherichia coli
J. Bacteriol.
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7285-7294
1996
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1
-
8
-
-
-
-
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3
1
-
1
-
1
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-
-
-
-
-
1
2
-
-
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-
-
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-
-
-
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1
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8
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3
1
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-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
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-
-
651629
Guzzo
Analysis of the region between ...
Escherichia coli
J. Bacteriol.
178
7295-7303
1996
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1
-
7
-
-
-
-
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1
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1
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1
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-
1
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1
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7
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1
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
651630
Lee
Inhibition of RecA-mediated cl ...
Escherichia coli
J. Bacteriol.
178
7304-7307
1996
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1
-
1
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-
-
-
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1
-
1
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1
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1
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
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-
-
653917
Peat
The UmuD' protein filament and ...
Escherichia coli
Structure
4
1401-1412
1996
-
-
1
1
-
-
-
-
-
-
-
1
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2
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1
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1
2
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1
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1
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1
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1
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-
-
-
1
2
-
-
-
-
-
-
-
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651622
Lee
A monocysteine approach for pr ...
Escherichia coli
J. Bacteriol.
176
4825-4837
1994
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-
-
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8
1
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-
-
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1
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1
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1
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1
1
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8
1
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1
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1
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-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
653236
Koch
Escherichia coli umuDC mutants ...
Escherichia coli
Mol. Gen. Genet.
233
443-448
1992
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1
-
3
-
-
-
-
-
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4
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1
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-
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1
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3
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1
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-
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-
-
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-
-
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-
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-
653235
Woodgate
Levels of chromosomally encode ...
Escherichia coli
Mol. Gen. Genet.
229
10-16
1991
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
1
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1
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1
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1
1
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-
1
-
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-
-
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-
-
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-
651617
Thomas
Structural characterization of ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
J. Bacteriol.
172
4979-4987
1990
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1
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1
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4
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1
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1
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-
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-
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-
653604
Battista
Dominant negative umuD mutatio ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
87
7190-7194
1990
-
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1
-
10
-
-
-
-
-
-
-
-
2
-
1
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-
-
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1
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1
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-
10
-
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1
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1
-
-
-
-
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-
-
-
-
-
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-
653602
Shinagawa
RecA protein-dependent cleavag ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
85
1806-1810
1988
-
-
1
-
-
-
-
-
-
-
2
1
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3
-
1
1
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-
-
-
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1
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-
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-
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-
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1
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-
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2
1
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1
1
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1
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-