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Literature summary for 3.4.21.B30 extracted from

  • Lee, M.H.; Walker, G.C.
    Interactions of Escherichia coli UmuD with activated RecA analyzed by cross-linking UmuD monocysteine derivatives (1996), J. Bacteriol., 178, 7285-7294.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B30

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
L44C 4-azidoiodoacetanilide-modified mutant, almost no cross-linking with RecA Escherichia coli
S112C 4-azidoiodoacetanilide-modified mutant, cross-links moderately efficiently with RecA Escherichia coli
S19C 4-azidoiodoacetanilide-modified mutant, almost no cross-linking with RecA Escherichia coli
S57C 4-azidoiodoacetanilide-modified mutant, cross-links moderately efficiently with RecA Escherichia coli
S60C 4-azidoiodoacetanilide-modified mutant, no cross-linking with RecA Escherichia coli
S67C 4-azidoiodoacetanilide-modified mutant, cross-links moderately efficiently with RecA Escherichia coli
S81C 4-azidoiodoacetanilide-modified mutant, cross-links most efficiently with RecA Escherichia coli
V34C 4-azidoiodoacetanilide-modified mutant, cross-links most efficiently with RecA Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 dimer, calculated from nucleic acid sequence Escherichia coli
40000
-
 dimer, SDS-PAGE Escherichia coli
72000
-
 cross-linked tetramers, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli interacts with RecA protein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification inactive form UmuD is cleaved into active form UmuD' Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information interacts with RecA protein Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
dimer UmuD can form homodimers, crosslinking experiments, SDS-PAGE Escherichia coli
tetramer two dimers form a tetramer, wild-type and V34C mutant, SDS-PAGE Escherichia coli