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Literature summary for 3.4.21.B28 extracted from

  • Edosada, C.Y.; Quan, C.; Tran, T.; Pham, V.; Wiesmann, C.; Fairbrother, W.; Wolf, B.B.
    Peptide substrate profiling defines fibroblast activation protein as an endopeptidase of strict Gly(2)-Pro(1)-cleaving specificity (2006), FEBS Lett., 580, 1581-1586.
    View publication on PubMed
Search for more literature for 3.4.21.B28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg 23°C Homo sapiens
0.0007
-
 Thr-Ala-Gly-Pro-Asn-Gln 23°C Homo sapiens
0.0008
-
 Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg 23°C Homo sapiens
0.0013
-
 Thr-Ser-Gly-Pro-Asn-Gln 23°C Homo sapiens
0.0019
-
 Thr-Ser-Gly-Pro-Ser-Gln 23°C Homo sapiens
0.0022
-
 Ala-Ser-Gly-Pro-Asn-Gln 23°C Homo sapiens
0.0022
-
 Thr-Ser-Gly-Pro-Asn-Ser 23°C Homo sapiens
0.0043
-
 Ala-Ser-Gly-Pro-Ser-Ser 23°C Homo sapiens
0.213
-
 D-Ala-Pro-7-amido-4-trifluoromethylcoumarin 23°C Homo sapiens
0.541
-
 acetyl-D-Ala-Pro-7-amido-4-trifluoromethylcoumarin 23°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q12884
-
-

Reaction

Reaction Comment Organism Reaction ID
fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Met-alpha2-antiplasmin is cleaved N-terminally at Pro3-Leu4 and Pro12-Asn13 into Asn-alpha2-antiplasmin that is rapidly cross-linked to fibrin and protects it from digestion by plasmin besides glycine, fibroblast activation protein tolerates substrates with D-Ala and D-Ser at P2. Fibroblast activation protein prefers small, uncharged amino acids at P3, but tolerates most amino acids at P4, P1' and P2' Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-D-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens acetyl-D-Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
Ala-Ser-Gly-Pro-Asn-Gln + H2O
-
 Homo sapiens Ala-Ser-Gly-Pro + Asn-Gln
-
 ?
Ala-Ser-Gly-Pro-Ser-Ser + H2O
-
 Homo sapiens Ala-Ser-Gly-Pro + Ser-Ser
-
 ?
Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg + H2O
-
 Homo sapiens Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg
-
 ?
Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg + H2O
-
 Homo sapiens Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg
-
 ?
D-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens D-Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
additional information fibroblast activation protein requires substrates with Pro at P1 and Gly or D-amino acids at P2. Besides glycine, fibroblast activation protein tolerates substrates with D-Ala and D-Ser at P2. Fibroblast activation protein prefers small, uncharged amino acids at P3, but tolerates most amino acids at P4, P1' and P2' Homo sapiens ?
-
 ?
Thr-Ala-Gly-Pro-Asn-Gln + H2O
-
 Homo sapiens Thr-Ala-Gly-Pro + Asn-Gln
-
 ?
Thr-Ser-Gly-Pro-Asn-Gln + H2O
-
 Homo sapiens Thr-Ser-Gly-Pro + Asn-Gln
-
 ?
Thr-Ser-Gly-Pro-Asn-Ser + H2O
-
 Homo sapiens Thr-Ser-Gly-Pro + Asn-Ser
-
 ?
Thr-Ser-Gly-Pro-Ser-Gln + H2O
-
 Homo sapiens Thr-Ser-Gly-Pro + Ser-Gln
-
 ?

Synonyms

Synonyms Comment Organism
FAP
-
 Homo sapiens
fibroblast activation protein
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ala-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg 23°C Homo sapiens
0.22
-
 Arg-Lys-(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-D-Ser-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid)-Arg 23°C Homo sapiens
0.3
-
 acetyl-D-Ala-Pro-7-amido-4-trifluoromethylcoumarin 23°C Homo sapiens
1.5
-
 Thr-Ala-Gly-Pro-Asn-Gln 23°C Homo sapiens
1.5
-
 D-Ala-Pro-7-amido-4-trifluoromethylcoumarin 23°C Homo sapiens
1.6
-
 Thr-Ser-Gly-Pro-Asn-Gln 23°C Homo sapiens
2
-
 Thr-Ser-Gly-Pro-Ser-Gln 23°C Homo sapiens
2.7
-
 Ala-Ser-Gly-Pro-Asn-Gln 23°C Homo sapiens
3.1
-
 Thr-Ser-Gly-Pro-Asn-Ser 23°C Homo sapiens
5.5
-
 Ala-Ser-Gly-Pro-Ser-Ser 23°C Homo sapiens