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Literature summary for 3.4.21.B28 extracted from
- Antiplasmin-cleaving enzyme is a soluble form of fibroblast activation protein (2006), Blood, 107, 1397-1404.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FAP is expressed in Pichia pastoris | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | pH 7.5, 22°C, APCE | Homo sapiens |  |
| 0.029 | - |
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 0.101 | - |
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 0.124 | - |
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 22°C, APCE | Homo sapiens | |
| 0.272 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 0.323 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.5, 22°C, APCE | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
2 * 93000, recombinant FAP, SDS-PAGE | Homo sapiens |
| 97000 | - |
2 * 97000, native APCE, SDS-PAGE | Homo sapiens |
| 153000 | - |
recombinant FAP, gel filtration | Homo sapiens |
| 175000 | - |
native APCE, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Met-alpha2-antiplasmin + H2O | Homo sapiens | APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4. Conversion of Met-alpha2-antiplasmin by membrane or soluble FAP to the more easily fibrin-incorporable form, Asn-alpha2-antiplasmin, may increase plasmin inhibition within fibrin surrounding certain neoplasms and have an impact on growth and therapeutic susceptibility | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O | - |
Homo sapiens | Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | - |
? | |
| benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| Met-alpha2-antiplasmin + H2O | APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4. Conversion of Met-alpha2-antiplasmin by membrane or soluble FAP to the more easily fibrin-incorporable form, Asn-alpha2-antiplasmin, may increase plasmin inhibition within fibrin surrounding certain neoplasms and have an impact on growth and therapeutic susceptibility | Homo sapiens | ? | - |
? | |
| Met-alpha2-antiplasmin + H2O | APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 93000, recombinant FAP, SDS-PAGE | Homo sapiens |
| dimer | 2 * 97000, native APCE, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| antiplasmin-cleaving enzyme | soluble form of fibroblast activation protein | Homo sapiens |
| APCE | soluble form of fibroblast activation protein | Homo sapiens |
| FAP | - |
Homo sapiens |
| fibroblast activation protein | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 0.54 | - |
benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 22°C, APCE | Homo sapiens | |
| 0.995 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | APCE | Homo sapiens | |
| 1.06 | - |
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | pH 7.5, 22°C, APCE | Homo sapiens | |
| 1.08 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 1.2 | - |
Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg | pH 7.5, 22°C, recombinant FAP | Homo sapiens | |
| 6.08 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | pH 7.5, 22°C, APCE | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
substrate: Arg-Lys(DABCYL)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(EDANS)-Arg, APCE or rFAP | Homo sapiens |
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