Cloned (Comment) | Organism |
---|---|
- |
Hepacivirus C |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | - |
Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.087 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.088 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.102 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.403 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C | |
0.49 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C | |
0.502 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C | |
0.519 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant single-chain protease | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-DEEMECASHLPYK-NH2 + H2O | - |
Hepacivirus C | Ac-DEEMEC + ASHLPYK-NH2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00167 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.00183 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C | |
0.0233 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.0253 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.0255 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT | Hepacivirus C | |
0.0267 | - |
Ac-DEEMECASHLPYK-NH2 | wild-type or engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C | |
0.03 | - |
Ac-DEEMECASHLPYK-NH2 | engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl | Hepacivirus C |