Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NS4A | enhances the activity of NS3 protease in all cleavages via the formation of an NS3/NS4A | Hepacivirus C | |
NS4A | hydrophobic central core of NS4A corresponding to residues 21-34, kinetic value for a modified peptide modified with a KK residue at the C-terminus to enhance solubility, Km: 0.00076 mM, kcat: 9 per min | Hepacivirus C |
Cloned (Comment) | Organism |
---|---|
residues 1027-1207 of the NS3 protein, expressed in Escherichia coli | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00076 | - |
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C | |
0.0028 | - |
acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C | |
0.0292 | - |
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | - |
Hepacivirus C | |
0.0436 | - |
7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18867 | - |
1 * 18867, NS3 protease domain residues from 1029-1206, mass spectrometry | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hepacivirus C |
Storage Stability | Organism |
---|---|
-80°C, 25 mM phosphate buffer, pH 7.5, 10% glycerol, 5 mM DTT | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) + H2O | - |
Hepacivirus C | 7-methoxycoumarin-4-acetyl-DEMEEC + ASHLPYK-(epsilon-NHCOCH3) | - |
? | |
7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe + H2O | - |
Hepacivirus C | 7-methoxycoumarin-4-acetyl-EDASTPC + SGSN-(4-nitro)Phe | - |
? | |
acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) + H2O | - |
Hepacivirus C | acetyl-DEMEEC + ASHLPYK-(epsilon-NHCOCH3) | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 18867, NS3 protease domain residues from 1029-1206, mass spectrometry | Hepacivirus C |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | - |
Hepacivirus C | |
0.143 | - |
acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C | |
0.15 | - |
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C | |
0.227 | - |
7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe | presence of inhibitor peptide 4Apep21-34KK | Hepacivirus C |