Protein Variants | Comment | Organism |
---|---|---|
A156S | resistant to inhibitor VX-950, sensitive to inhibitor BILN 2061 | Hepacivirus C |
A156T | resistant to both inhibotors VX-950 and BILN 2061, diminished replication capacity in a transient replicon cell system | Hepacivirus C |
A156V | resistant to both inhibotors VX-950 and BILN 2061, diminished replication capacity in a transient replicon cell system | Hepacivirus C |
D168A | sensitive to inhibitor VX-950, resistant to inhibitor BILN 2061 | Hepacivirus C |
D168V | sensitive to inhibitor VX-950, resistant to inhibitor BILN 2061 | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BILN 2061 | - |
Hepacivirus C | |
VX-950 | - |
Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | wild-type, pH 7.8, 30°C | Hepacivirus C | |
1.3 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | mutant A156T, pH 7.8, 30°C | Hepacivirus C | |
2.6 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | mutant A156V, pH 7.8, 30°C | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | P26663 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 + H2O | - |
Hepacivirus C | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | mutant A156T, pH 7.8, 30°C | Hepacivirus C | |
0.6 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | mutant A156V, pH 7.8, 30°C | Hepacivirus C | |
1 | - |
Ac-L-Asp-L-Glu-L-Asp-(EDANS)-L-Glu-L-Glu-alpha-aminobutyric acid-PSI[COO]-L-Ala-L-Ser-L-Lys-(DABCYL)-NH2 | wild-type, pH 7.8, 30°C | Hepacivirus C |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000019 | - |
BILN 2061 | wild-type, pH 7.8, 30°C | Hepacivirus C | |
0.0001 | - |
VX-950 | wild-type, pH 7.8, 30°C | Hepacivirus C | |
0.0012 | - |
BILN 2061 | or above, mutant A156T, pH 7.8, 30°C | Hepacivirus C | |
0.0012 | - |
BILN 2061 | or above, mutant A156V, pH 7.8, 30°C | Hepacivirus C | |
0.0099 | - |
VX-950 | mutant A156T, pH 7.8, 30°C | Hepacivirus C | |
0.033 | - |
VX-950 | mutant A156V, pH 7.8, 30°C | Hepacivirus C |