Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.98 extracted from

  • Chappell, K.J.; Nall, T.A.; Stoermer, M.J.; Fang, N.X.; Tyndall, J.D.; Fairlie, D.P.; Young, P.R.
    Site-directed mutagenesis and kinetic studies of the West Nile Virus NS3 protease identify key enzyme-substrate interactions (2005), J. Biol. Chem., 280, 2896-2903.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D129A 15fold increase in KM-value compared to wild-type West Nile virus
D129E 3fold increase in KM-value compared to wild-type West Nile virus
D129N 3fold increase in KM-value compared to wild-type West Nile virus
N152A inactive West Nile virus
S135A inactive West Nile virus
S160A 2fold increase in KM-value compared to wild-type West Nile virus
S163A inactive West Nile virus
V115A 2fold increase in KM-value compared to wild-type West Nile virus
V115F inactive West Nile virus
Y150A inactive West Nile virus
Y150F 50% increase in KM-value compared to wild-type West Nile virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
Ac-KPGLKR-4-nitroanilide wild-type, pH 9.5, 37°C West Nile virus
0.246
-
Ac-KPGLKR-4-nitroanilide mutant Y150F, pH 9.5, 37°C West Nile virus
0.357
-
Ac-KPGLKR-4-nitroanilide mutant S160A, pH 9.5, 37°C West Nile virus
0.36
-
Ac-KPGLKR-4-nitroanilide mutant V115A, pH 9.5, 37°C West Nile virus
0.561
-
Ac-KPGLKR-4-nitroanilide mutant D129E, pH 9.5, 37°C West Nile virus
0.611
-
Ac-KPGLKR-4-nitroanilide mutant D129N, pH 9.5, 37°C West Nile virus
2.942
-
Ac-KPGLKR-4-nitroanilide mutant D129A, pH 9.5, 37°C West Nile virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE West Nile virus

Organism

Organism UniProt Comment Textmining
West Nile virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-KPGLKR-4-nitroanilide + H2O
-
West Nile virus ?
-
?
additional information substrate P1 R residue strongly interacts with S1 residues D129, Y150, and S163. P2 K residue makes an essential interaction with N152 at S2 West Nile virus ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE West Nile virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
Ac-KPGLKR-4-nitroanilide mutant D129A, pH 9.5, 37°C West Nile virus
0.019
-
Ac-KPGLKR-4-nitroanilide mutant D129E, pH 9.5, 37°C West Nile virus
0.038
-
Ac-KPGLKR-4-nitroanilide mutant D129N, pH 9.5, 37°C West Nile virus
0.133
-
Ac-KPGLKR-4-nitroanilide mutant Y150F, pH 9.5, 37°C West Nile virus
0.394
-
Ac-KPGLKR-4-nitroanilide mutant S160A, pH 9.5, 37°C West Nile virus
0.872
-
Ac-KPGLKR-4-nitroanilide mutant V115A, pH 9.5, 37°C West Nile virus
0.965
-
Ac-KPGLKR-4-nitroanilide wild-type, pH 9.5, 37°C West Nile virus
6.08
-
Ac-KPGLKR-4-nitroanilide mutant V115A, pH 9.5, 37°C West Nile virus
6.08
-
Ac-KPGLKR-4-nitroanilide wild-type, pH 9.5, 37°C West Nile virus