Protein Variants | Comment | Organism |
---|---|---|
D129A | 15fold increase in KM-value compared to wild-type | West Nile virus |
D129E | 3fold increase in KM-value compared to wild-type | West Nile virus |
D129N | 3fold increase in KM-value compared to wild-type | West Nile virus |
N152A | inactive | West Nile virus |
S135A | inactive | West Nile virus |
S160A | 2fold increase in KM-value compared to wild-type | West Nile virus |
S163A | inactive | West Nile virus |
V115A | 2fold increase in KM-value compared to wild-type | West Nile virus |
V115F | inactive | West Nile virus |
Y150A | inactive | West Nile virus |
Y150F | 50% increase in KM-value compared to wild-type | West Nile virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Ac-KPGLKR-4-nitroanilide | wild-type, pH 9.5, 37°C | West Nile virus | |
0.246 | - |
Ac-KPGLKR-4-nitroanilide | mutant Y150F, pH 9.5, 37°C | West Nile virus | |
0.357 | - |
Ac-KPGLKR-4-nitroanilide | mutant S160A, pH 9.5, 37°C | West Nile virus | |
0.36 | - |
Ac-KPGLKR-4-nitroanilide | mutant V115A, pH 9.5, 37°C | West Nile virus | |
0.561 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129E, pH 9.5, 37°C | West Nile virus | |
0.611 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129N, pH 9.5, 37°C | West Nile virus | |
2.942 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129A, pH 9.5, 37°C | West Nile virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | West Nile virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
West Nile virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-KPGLKR-4-nitroanilide + H2O | - |
West Nile virus | ? | - |
? | |
additional information | substrate P1 R residue strongly interacts with S1 residues D129, Y150, and S163. P2 K residue makes an essential interaction with N152 at S2 | West Nile virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | West Nile virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129A, pH 9.5, 37°C | West Nile virus | |
0.019 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129E, pH 9.5, 37°C | West Nile virus | |
0.038 | - |
Ac-KPGLKR-4-nitroanilide | mutant D129N, pH 9.5, 37°C | West Nile virus | |
0.133 | - |
Ac-KPGLKR-4-nitroanilide | mutant Y150F, pH 9.5, 37°C | West Nile virus | |
0.394 | - |
Ac-KPGLKR-4-nitroanilide | mutant S160A, pH 9.5, 37°C | West Nile virus | |
0.872 | - |
Ac-KPGLKR-4-nitroanilide | mutant V115A, pH 9.5, 37°C | West Nile virus | |
0.965 | - |
Ac-KPGLKR-4-nitroanilide | wild-type, pH 9.5, 37°C | West Nile virus | |
6.08 | - |
Ac-KPGLKR-4-nitroanilide | mutant V115A, pH 9.5, 37°C | West Nile virus | |
6.08 | - |
Ac-KPGLKR-4-nitroanilide | wild-type, pH 9.5, 37°C | West Nile virus |