Literature summary for 3.4.21.98 extracted from

Hamill, P.; Jean, F.
Enzymatic characterization of membrane-associated hepatitis C virus NS3-4A heterocomplex serine protease activity expressed in human cells (2005), Biochemistry, 44, 6586-6596.

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Inhibitors

Inhibitors	Comment	Organism	Structure
fluorescein-L-Asp-D-Glu-L-Leu-L-Ile-L-beta-cyclohexylalanine-L-Cys-L-Pro-L-beta-cyclohexylalanine-L-Asp-L-Leu-NH2	i.e. PepInh-1, noncleavable decapeptidyl inhibitor	Hepacivirus C

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	o-aminobenzoyl (Abz)-L-Asp-L-Asp-L-Ile-L-Val-L-Pro-L-Cys-L-Ser-L-Met-L-Ser-L-Tyr(3-NO2)-L-Thr-NH2	pH 7.3, 30°C	Hepacivirus C

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	membrane anchoring of enzyme does not affect substrate/inhibitor recognition	Hepacivirus C	16020	-

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	expression in human osteosarcoma cells	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	specific and sensitive assay using an internally quenched fluorogenic substrate	Hepacivirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
o-aminobenzoyl (Abz)-L-Asp-L-Asp-L-Ile-L-Val-L-Pro-L-Cys-L-Ser-L-Met-L-Ser-L-Tyr(3-NO2)-L-Thr-NH2 + H2O	i.e. IQGS-1, internally quenched fluorogenic substrate	Hepacivirus C	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0065	-	o-aminobenzoyl (Abz)-L-Asp-L-Asp-L-Ile-L-Val-L-Pro-L-Cys-L-Ser-L-Met-L-Ser-L-Tyr(3-NO2)-L-Thr-NH2	pH 7.3, 30°C	Hepacivirus C

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000124	-	fluorescein-L-Asp-D-Glu-L-Leu-L-Ile-L-beta-cyclohexylalanine-L-Cys-L-Pro-L-beta-cyclohexylalanine-L-Asp-L-Leu-NH2	pH 7.3, 30°C	Hepacivirus C

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Release 2023.1 (January 2023)