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Literature summary for 3.4.21.98 extracted from

  • Beyer, B.M.; Zhang, R.; Hong, Z.; Madison, V.; Malcolm, B.A.
    Effect of naturally occurring active site mutations on hepatitis C virus NS3 protease specificity (2001), Proteins Struct. Funct. Genet., 43, 82-88.
    View publication on PubMed
Search for more literature for 3.4.21.98

Protein Variants

Protein Variants Comment Organism
D168Q mutation in strain 1a, mutation has no impact on inhibition profile Hepacivirus C
Q168D mutation in strain 3a, mutation has no impact on inhibition profile Hepacivirus C
R123T mutation in strain 1a, inhibition profile is identical to that of enzyme from 3a strain Hepacivirus C
R123T/D168Q mutation in strain 1a, inhibition profile is identical to that of enzyme from 3a strain Hepacivirus C
T123R mutation in strain 3a, inhibition profile is identical to that of enzyme from 1a strain Hepacivirus C
T123R/Q168D mutation in strain 3a, inhibition profile is identical to that of enzyme from 1a strain Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH
-
 Hepacivirus C
acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH
-
 Hepacivirus C
acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH
-
 Hepacivirus C
acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH
-
 Hepacivirus C
acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH
-
 Hepacivirus C
acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH
-
 Hepacivirus C
acetyl-DEMEEC-OH
-
 Hepacivirus C
acetyl-DYPSPC-OH
-
 Hepacivirus C
acetyl-ECTTPC-OH
-
 Hepacivirus C
acetyl-EDVVCC-OH
-
 Hepacivirus C
acetyl-QSVVCC-OH
-
 Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
 genotype 1a, 1b, and 3a
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-DTEDVVP-norvaline-O-4-phenylazophenol + H2O
-
 Hepacivirus C ?
-
 ?
viral polyprotein + H2O
-
 Hepacivirus C ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0011
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.0012
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys pH 6.5, 30°C, strain 1a Hepacivirus C
0.0018
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.0022
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.0026
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.0027
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.0028
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.0029
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R Hepacivirus C
0.0032
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R Hepacivirus C
0.0032
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R Hepacivirus C
0.0033
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.004
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.0045
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.005
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.0052
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.009
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.01
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.01
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.01
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.01
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.013
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.013
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R Hepacivirus C
0.015
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.016
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.018
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.018
-
 acetyl-Asp-(D-Glu)-Tyr-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.018
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.019
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.019
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R Hepacivirus C
0.02
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.02
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.02
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.02
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.02
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.021
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.021
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.021
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.022
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.023
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.023
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.023
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.024
-
 acetyl-Asp-(D-homoleucine)-Leu-Ile-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme D168Q Hepacivirus C
0.024
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.024
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T12 3R Hepacivirus C
0.025
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.025
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.025
-
 acetyl-Asp-(D-Glu)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.025
-
 acetyl-Asp-(D-glutamine)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme T123R/Q168D Hepacivirus C
0.03
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.03
-
 acetyl-Asp-(D-homoglutamate)-Leu-Ile-L-cyclohexylalanine-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.04
-
 acetyl-QSVVCC-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.04
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.04
-
 acetyl-QSVVCC-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.045
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 3a, mutant enzyme Q168D Hepacivirus C
0.05
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T Hepacivirus C
0.05
-
 acetyl-QSVVCC-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.055
-
 acetyl-Asp-(D-Glu)-(allyloxycarbonyl-L-lysine)-(L-cyclohexylalanine)-Cys-OH pH 6.5, 30°C, strain 1a, mutant enzyme R123T/D168Q Hepacivirus C
0.1
-
 acetyl-EDVVCC-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.13
-
 acetyl-EDVVCC-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.15
-
 acetyl-EDVVCC-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.17
-
 acetyl-DEMEEC-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.2
-
 acetyl-DEMEEC-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.21
-
 acetyl-DEMEEC-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.4
-
 acetyl-DYPSPC-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.43
-
 acetyl-DYPSPC-OH pH 6.5, 30°C, strain 3a Hepacivirus C
0.45
-
 acetyl-DYPSPC-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.46
-
 acetyl-ECTTPC-OH pH 6.5, 30°C, strain 1b Hepacivirus C
0.5
-
 acetyl-ECTTPC-OH pH 6.5, 30°C, strain 1a Hepacivirus C
0.51
-
 acetyl-ECTTPC-OH pH 6.5, 30°C, strain 3a Hepacivirus C