Literature summary for 3.4.21.97 extracted from

Khayat, R.; Batra, R.; Massariol, M.J.; Lagace, L.; Tong, L.
Investigating the role of histidine 157 in the catalytic activity of human cytomegalovirus protease (2001), Biochemistry, 40, 6344-6351.

Search for more literature for 3.4.21.97

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, wild-type and mutant enzymes	Human betaherpesvirus 5

Protein Variants

Protein Variants	Comment	Organism
A143Q/H157A	the ratio of turnover number to Km-value is 22fold lower than that of the wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5
A143Q/H157E	the ratio of turnover number to Km-value is 11.7fold lower than that of the wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5
A143Q/H157Q	the ratio of turnover number to Km-value is 19fold lower than that of the wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5
S134A/A143Q	the ratio of turnover number to Km-value is 150fold lower than that of the wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5
S134A/A143Q/H157A	the ratio of turnover number to Km-value is 174fold lower than that of the wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0101	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5
0.0145	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157E	Human betaherpesvirus 5
0.0203	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157Q	Human betaherpesvirus 5
0.0218	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157A	Human betaherpesvirus 5
0.0277	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme S134A/A143Q	Human betaherpesvirus 5
0.0356	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme S134A/A143Q/H157A	Human betaherpesvirus 5

Organism

Organism	UniProt	Comment	Textmining
Human betaherpesvirus 5	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid + H2O	-	Human betaherpesvirus 5	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala + Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00061	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme S134A/A143Q	Human betaherpesvirus 5
0.00069	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme S134A/A143Q/H157A	Human betaherpesvirus 5
0.0033	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157A	Human betaherpesvirus 5
0.0036	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157Q	Human betaherpesvirus 5
0.0041	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, mutant enzyme A143Q/H157E	Human betaherpesvirus 5
0.033	-	4-(4'-dimethylaminophenylazo)benzoyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-5-((2'-aminoethyl)amino)-naphthalene-1-sulfonic acid	pH 7.2, 30°C, wild-typelike mutant enzyme A143Q	Human betaherpesvirus 5

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Release 2023.1 (January 2023)