Application | Comment | Organism |
---|---|---|
medicine | Pc1 N222D homozygous mice are obese and mimic humans with mutations in PC1. This mutation will be a valuable resource in understanding the role of prohormone processing in energy homeostasis | Mus musculus |
Cloned (Comment) | Organism |
---|---|
human PC1 cDNA (amino acids 28-753) amplified, His-tagged and cloned into the MluI and NotI sites of a modified pFASTBAC1 expression vector. Wild-type and mutant N222D human PC1 expressed using baculovirus to infect Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N222D | autocatalytic and neuropeptide processing is impaired | Homo sapiens |
N222D | leads to obesity, abnormal proinsulin processing, reduced fecundity, impaired autocatalysis and multiple endocrinological defects in mice homozygous for the mutation. Increased energy intake, a more efficient metabolism and reduced alpha-MSH signaling contribute to the obesity. Heterozygous littermates exhibit an intermediate phenotype for both sexes, thus this mutation results in a semi-dominant phenotype | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
Western blotting, intramolecular autocatalytic cleaved 85 kDa isoform | Homo sapiens |
66000 | - |
Western blotting, intramolecular autocatalytically cleaved 85 kDa isoform | Mus musculus |
75000 | - |
Western blotting, most likely a processing intermediate of PC1 | Homo sapiens |
85000 | - |
Western blotting | Mus musculus |
85000 | - |
Western blotting | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
C57BL/6 mice | - |
Mus musculus C57BL/6 | - |
C57BL/6 mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | 66 kDa and 85kDa isoforms | Mus musculus | - |
hypothalamus | - |
Mus musculus | - |
pancreas | 66 kDa isoform | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pGlu-Arg-Thr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PC1 | - |
Mus musculus |
PC1 | - |
Homo sapiens |
prohormone convertase 1 | - |
Mus musculus |
prohormone convertase 1 | - |
Homo sapiens |