Literature summary for 3.4.21.93 extracted from

Rabah, N.; Gauthier, D.; Dikeakos, J.D.; Reudelhuber, T.L.; Lazure, C.
The C-terminal region of the proprotein convertase 1/3 (PC1/3) exerts a bimodal regulation of the enzyme activity in vitro (2007), FEBS J., 274, 3482-3491.

Search for more literature for 3.4.21.93

Activating Compound

Activating Compound	Comment	Organism	Structure
PC1/3 CT peptide	increases PC1/3 activity at low concentrations (nanomol range). Interaction with the active site of PC1/3 does not result in the formation of a stable complex. Activation of PC1/3 by 5 nanomol CT-peptide is the same in the presence or absence of propeptide	Mus musculus

Application

Application	Comment	Organism
additional information	PC1/3, through its various domains, is capable of controlling its enzymatic activity in all regions of the cell that it encounters	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
PC1/3 produced using the baculovirus expression system in Sf9 insect cells or through intracoelemic injection in insect larvae. PC1/3 CT-peptide from positions 592-726 cloned into a pet24b+ bacterial expression vector. The resulting C-terminally His-tagged protein expressed in Escherichia coli strain BL21 (DE3)	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
PC1/3 CT peptide	decreases PC1/3 activity at high concentrations (micromol range)	Mus musculus
PC1/3 propeptide	20 nanomol leads to a 50% reduction in enzymatic activity	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17640	-	mass spectrometry	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PC1/3 purified. PC1/3 CT peptide purified by His-affinity chromatography and RP-HPLC	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	PC1/3 CT peptide is not cleaved by enzymatically active PC1/3	Mus musculus	?	-	?
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O	-	Mus musculus	pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
PC1/3	-	Mus musculus
proprotein convertase 1/3	-	Mus musculus

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	at pH 7.8 increase of enzymatic activity up to 50-60%, irrespective of the amount of PC1/3 CT peptide used up to 0.005 mM	Mus musculus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.002	-	PC1/3 CT peptide	-	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)