Protein Variants | Comment | Organism |
---|---|---|
D65A | site-directed mutagenesis of the propeptide residue, leads to reduced inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
D66A | site-directed mutagenesis of the propeptide residue, leads to reduced inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
D67A | site-directed mutagenesis of the propeptide residue, leads to increased inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
K61A | site-directed mutagenesis of the propeptide residue, leads to reduced inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
additional information | single amino acid substitution in the PC1/3 propeptide can induce significant modifications of its inhibitory profile toward its cognate enzyme | Mus musculus |
R50A | site-directed mutagenesis of the propeptide residue, leads to increased inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
R51A | site-directed mutagenesis of the propeptide residue, leads to increased inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
R53A | site-directed mutagenesis of the propeptide residue, leads to reduced inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
R54A | site-directed mutagenesis of the propeptide residue, leads to increased inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
R62A | site-directed mutagenesis of the propeptide residue, leads to reduced inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
S52A | site-directed mutagenesis of the propeptide residue, unaltered inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
S52A/R53A | site-directed mutagenesis of the propeptide residues, leads to increased inhibition of mature PC1 by the separated propeptide mutant compared to the wild-type propeptide | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PC1/3 propeptide | inhibits the mature enzyme competitively, inhibition mechanism, interaction with the enzyme at the 50RRSRR54, 61KR62 and 65DDD67 sequences, molecular modeling, overview | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P63239 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PC1/3 | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000015 | - |
mutant D67A propeptide | pH 6.0, 22°C | Mus musculus | |
0.0000002 | - |
mutant R54A propeptide | pH 6.0, 22°C | Mus musculus | |
0.00000036 | - |
mutant R50A propeptide | pH 6.0, 22°C | Mus musculus | |
0.00000074 | - |
mutant R51A propeptide | pH 6.0, 22°C | Mus musculus | |
0.000004 | - |
mutant R52A propeptide | pH 6.0, 22°C | Mus musculus | |
0.0000044 | - |
wild-type propeptide | pH 6.0, 22°C | Mus musculus | |
0.0000054 | - |
mutant R53A propeptide | pH 6.0, 22°C | Mus musculus | |
0.0000086 | - |
mutant D66A propeptide | pH 6.0, 22°C | Mus musculus | |
0.00001 | - |
mutant K61A propeptide | pH 6.0, 22°C | Mus musculus | |
0.00001 | - |
mutant D65A propeptide | pH 6.0, 22°C | Mus musculus | |
0.0000165 | - |
mutant R62A propeptide | pH 6.0, 22°C | Mus musculus |