Protein Variants | Comment | Organism |
---|---|---|
A153C | the structure of a crosslinked Escherichia coli ClpP are determined in which the two heptameric rings of ClpP are held together by disulfide bonds. While all Escherichia coli ClpP structures solved to date are in the extended state, the crosslinked ClpP structure is found to be in the compact state. Under reducing condition Km (N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin) is similar to wild-type but kcat is 32fold lower. Under non-reducing conditions mutant is inactive and does not bind its cognate chaperone | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, non-reducing conditions, 4°C, pH not specified in the publication | Escherichia coli | |
1 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, reducing conditions, 4°C, pH not specified in the publication | Escherichia coli | |
1.1 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | mutant A153C, reducing conditions, 4°C, pH not specified in the publication | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Escherichia coli |
346000 | - |
sedimentation velocity analytical ultracentrifugation, tetradecamer | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Escherichia coli | N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | tetradecamer, sedimentation velocity analytical ultracentrifugation | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ClpP | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | mutant A153C, reducing conditions, 4°C, pH not specified in the publication | Escherichia coli | |
9.6 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, non-reducing conditions, 4°C, pH not specified in the publication | Escherichia coli | |
15.8 | - |
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin | wild-type, reducing conditions, 4°C, pH not specified in the publication | Escherichia coli |