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Literature summary for 3.4.21.92 extracted from

  • Hwang, B.J.; Woo, K.M.; Goldberg, A.L.; Chung, C.H.
    Protease Ti, a new ATP-dependent protease in Escherichia coli, contains protein-activated ATPase and proteolytic functions in distinct subunits (1988), J. Biol. Chem., 263, 8727-8734.
    View publication on PubMed

General Stability

General Stability Organism
Repeated freezing and thawing leads to loss of activity of subunit ClpA Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate inactivates ClpP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
ATP for hydrolysis of both casein and ATP Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ poor substitute for Mg2+ Escherichia coli
Mg2+ optimal concentration: 5.1 mM Escherichia coli
Mn2+ can partially substitute for Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
80000
-
x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
340000
-
E. coli Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
RGC125 (lon-)
-
Escherichia coli RGC125 (lon-)
-
RGC125 (lon-)
-

Purification (Commentary)

Purification (Comment) Organism
ClpA Escherichia coli
ClpP Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-30°C, subunit ClpA, 1 month, 50% loss of activity Escherichia coli
-30°C, subunit ClpA, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 1 month, 50% loss of activity, Escherichia coli
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, subunit ClpA stable for several months Escherichia coli
4°C, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, 20% v/v glycerol, 80% loss of activity after 1 week, subunit ClpA Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Escherichia coli small peptides derived from casein
-
?
casein + H2O
-
Escherichia coli RGC125 (lon-) small peptides derived from casein
-
?

Subunits

Subunits Comment Organism
? x * 80000 (ClpA, SDS-PAGE, behaves as a dimer of MW 140000 Da on gel filtration) + x * 23000 (ClpP, SDS-PAGE, behaves as a complex of 10-12 subunits, MW 260000 Da) Escherichia coli
More enzyme consists of two components: ClpP and ClpA or ClpX Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
10 min, 20 mM Tris-HCl, pH 7.8, 5 mM MgCl2, 0.5 mM EDTA, 10% glycerol, little or no loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
7.0 (ClpA) Escherichia coli
additional information
-
8.2 (ClpP) Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP proteolytic activity Escherichia coli
dATP proteolytic activity Escherichia coli